MDCK-MDR1 permeability assay: Cyprotex’s MDCK-MDR1 cells originate from the transfection of Madin Darby Canine Kidney (MDCK) cells with the human MDR1 gene (ABCB1)3, the gene encoding for the efflux transporter, human P-gp. Assessing flux in both directions (apical to basolateral (A-B)...
Creative Bioarrayis a reliable MDR1-MDCK permeability assay provider. After years of development,Creative Bioarrayhas become an expert in drug discovery and development. Using a highly automated approach, we offer this in vitro ADME screening service to determine drug permeability. ...
One Assay, Two Vital Values‐Estimating Intrinsic Permeability and Efflux Using MDCK‐MDR1 Assaydoi:10.1096/fasebj.2022.36.S1.R2681Zang, GraceLi, ChunFASEB Journal
SakuraiA,et [7] I,GottesmanMM,UedaK,et anMDRleDNAconfers resistanceand human activity carrying multidrug invitro of inMDCKcells method with polarizedexpressionP·glyeoprotein assay by comparing transport ·564· PharmaceutieaSinica 药学学报Acta 2008,43(6):559—564 michaelis—Mentenkinetic to ...
METHODS:The inhibitory effect of HY2006 on pathologic changes caused by influenza virus on MDCK cell line was observed by micro-cytopathic inhibiting assay,and the inhibitory effect of HY2006 on influenza virus was investigated by chick-embryo culture technique. 方法:通过微量细胞病变抑制法观察HY2006...
and VER served as a positive control for the drug stimulation of P-gp ATPase activity. The effect of a compound was considered to be stimulatory if the attained ATPase activity was higher than the basal P-gp ATPase activity incubated with the pure assay buffer. Samples were measured in tripli...
Methods: The cytotoxicity of drugs on MDCK and MDCK-MDR1 was evaluated by the MTT assay, and the transport studies were performed in both directions. The membrane fluidity was evaluated by fluorescence recovery after photobleaching, and the membrane potential was estimated by the accumulation of ...
Monolayer transport assay across the MDCK-MDR1 cells.Zheyi, HuZaigang, ZhouYahui, HuJinhui, WuYunman, LiWenlong, Huang
Conclusion: The in vitro assay setup developed in this study holds promise for predicting in vivo drug brain penetration in CNS drug discovery. The correlation between in vitro and in vivo Kp,brain values, underscores that the model may have potential for early-stage ...
Calcein-AM assay was found to be non-predictive to surfactants influence on digoxin permeability across cell monolayers. In conclusion, these results may assist, in a mechanism-based, selection of suitable surfactants for formulating oral dosage forms to enhance the absorption of low bioavailable P-...