Single-Cell RNA Sequencing of Developing Ears Facilitates Functional Analysis and Trait Candidate Gene Discovery in MaizeMaize earmeristemscRNA-seqdevelopmental networksGWAStrait genesCrop productivity depends on activity of meristems that produce optimized plant architectures, including that of the maize ear...
We further identified differences in gene expression between the mitotic and meiotic cell cycles. Our results uncover a multi-step pathway into meiosis and highlight the power of single cell RNA-seq to define developmental transitions. 展开
This transcriptome study highlights genes exclusively and preferentially expressed in maize root hairs and their suggested functions, and provides a starting point for future genetic analyses. Root hairs are tubular extensions of epidermis cells. Transcriptome profiling demonstrated that the single cell-type...
To generate a single-cell atlas from differentiating endosperm, we harvested maize endosperm tissues at 6 and 7 DAP and digested them into protoplasts. We constructed four scRNA-seq libraries (one sample from 6 DAP and three replicates from 7 DAP) using the 10x Genomics Chromium platform, which...
The plots were single-row in the tropical region and the Gansu temperate region, and double-row in the Beijing temperate region. Flowering time was calculated as the number of days from planting until half of the plants in a row flowered. DTA and DTS were recorded for each inbred line. ...
and the use of DNase I treatment to remove any genomic DNA contamination from RNA samples. About 2 μg of total RNA was reverse transcribed using a Takara RNA Kit to generate the single-stranded cDNA. Real-time PCR was performed on iCycler iQ5 Multicolor real-time PCR detection system (Bio...
The WRKY transcription factor family plays significant roles in biotic and abiotic stress responses, which has been associated with various biological processes in higher plants. However, very little is known regarding the structure and function of WRKY
RNA quality control, library preparation, and sequencing were performed on an Illumina Novaseq 6000 platform at Novogene. Trimmomatic (version 0.38) [76] was used to trim the adaptor sequence and low-quality bases of RNA-Seq raw reads. The parameters used for the trimming is “ILLUMINACLIP:...
In 2), single QTL-paired materials are screened, planted, and sampled for RNA-seq. Identified DEGs and sequence variants are considered as proportional weights during candidate gene prediction. In 3), SD and ML algorithms are integrated into QTG-Miner. Using SD and ML algorithms, candidate ...
Plant Cell 25, 3640–3656 (2013). Article Google Scholar Denoeud, F. et al. Annotating genomes with massive-scale RNA sequencing. Genome Biol. 9, R175 (2008). Article Google Scholar Gordon, S. P. et al. Widespread polycistronic transcripts in fungi revealed by single-molecule mRNA ...