通过标准曲线校准计算得到m6A的含量.结果m6A-SAC-seq处理后,HIV逆转录酶识别环化的am6A位点,引入突变,但附近未修饰位点不发生突变.通过特定的A突变成T/C的突变位置(A to T/C)来识别m6A位点,并添加内参探针,用标准曲线来定量m6A含量.结论m6A-SAC-seq技术仅需30 ng的mRNA或去除了rRNA的总RNA样本就可以实现m6A...
For example, using apptainer run -B /data docker://sacseq:latest... default settings(Click to expand) default config file: data.yaml default output dir: ./results default jobs in parallel: 48 Read the documentation on how to customize. View the analytics report and use the m6A sites ...