我们提出了一种定量的m6A-SAC-seq方法,可在单碱基分辨率下绘制整个转录组中的m6A位点。该方法只需要~30ng的输入RNA (来自300ng的总RNA),因此适用于研究各种生物系统。我们以单碱基精度和化学计量信息绘制了详细的m6A综合图。m6A-SAC-se...
m6A-SAC-seq for quantitative whole transcriptome m6A profiling. Nat. Protoc. 18, 626–657 (2023). Article Google Scholar Chen, H.-X., Zhang, Z., Ma, D.-Z., Chen, L.-Q. & Luo, G.-Z. Mapping single-nucleotide m6A by m6A-REF-seq. Methods 203, 392–398 (2022). Article ...
Ge, R. et al. m6A-SAC-seq for quantitative whole transcriptome m6A profiling.Nat. Protoc.18, 626–657 (2023). ArticleGoogle Scholar Chen, H.-X., Zhang, Z., Ma, D.-Z., Chen, L.-Q. & Luo, G.-Z. Mapping single-nucleotide m6A by m6A-REF-seq.Methods203, 392–398 (2022). Ar...
m6A-SAC-seq for quantitative whole transcriptome m6A profiling Article 25 November 2022 Data availability The data that support the findings of this study have been deposited in NCBI’s Gene Expression Omnibus under accession code GSE125780. References Zhao, B. S., Roundtree, I. A. & He,...
🧪 Optimized protocol for m6A-SAC-seq dockerpipelinengsrnamodificationm6aepitranscriptomicsepitranscriptomesac-seqsingle-base UpdatedOct 6, 2024 Python chanzhou/AutoCirc Star6 Code Issues Pull requests A computational toolkit of fast and automatical identification of back-splice junctions of potential circul...
One of the latest m6A technology is the m6A-Selective Allyl Chemical labelling and sequencing or m6A-SAC-seq. This method is based on the Dim1/KsgA dimethyltransferases, which transfer the methyl-group from SAM to adenosines, forming initially m6A and m62A in a constitutive methylation reaction...
Hepatic specification and functional maturation are tightly controlled throughout development. N6-methyladenosine (m6A) is the most abundant RNA modification of eukaryotic mRNAs and is involved in various physiological and pathological processes. However
mA-SAC-seq for quantitative whole transcriptome m6 Introduction Eukaryotic mRNA is canonically modified by the addition of the 5ʹ cap and bears additional modifications at internal sites. TheN6-methylation of adenosine (m6A) is the most abundant and best-studied internal modification of mRNA. It...
The miRNA-seq or m6A-related lncRNA analy- sis revealed that HNRNPA2B1 can act by regulating miRNAs and lncRNAs in cancer [27, 28]. In the present study we found that HNRNPA2B1 upregulation was associated with distant metastasis, acting as an independ- ent prognostic factor for poor ...