Light sheet microscopySelect plane illumination microscopyBessel beamLattice light sheet microscopyWhole organism imagingCapturing dynamic processes in live samples is a nChewHowardTeng-LeongHowardHeddlestonHowardJohnHowardM.HowardThe international journal of biochemistry and cell biology...
1.ZEISS Lightsheet Z.1(Light Sheet Fluorescence Microscopy for Multiview Imaging of Large Specimens)下载 2.Application Note:(Improved Imaging of Cleared Samples with ZEISS Lightsheet Z.1)下载 3.Application Note:(Fast Imaging of Cellular Spheroids with Light Sheet Fluorescence Microscopy)下载 4.Appli...
Light-sheet microscopes enable rapid high-resolution imaging of biological specimens; however, biological processes span spatiotemporal scales. Moreover, long-term phenotypes are often instigated by rare or fleeting biological events that are difficult t
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为了解决这个问题Hari Schroff教授在2011年发明了Dual Illumination Selective Plan Imaging Microscopy (diSPIM)技术,并在2013年年将此技术发表在了Nature Biotechnology上,这在后来成为了3i显微镜公司的Marianas Lightsheet Microscope(MLS光片显微镜)。 Fig.2 传统光片显微镜的上样需将样品放入一个竖着的玻璃...
Light-sheet microscopy uses a thin plane of light to illuminate a sample orthogonally to the detection objective such that the axial resolution of the microscope is determined wholly or partly by the thickness of the light sheet. It allows fast volumetric imaging with reduced sample irradiation comp...
Light sheet microscopyOptical clearingStem cell nichesRegenerative fociOptical clearing combined with deep imaging of large biological specimen allows organ-wide visualization of cells in three dimensions (3D) to explore regenerative processes in a spatial context. Here, we investigate the dynamics of ...
The entire development of the nervous system can now be viewed with excellent temporal resolution using the exceptional capture rate of the Neo sCMOS combined with the Light Sheet Microscopy technique.Philipp Keller and his colleagues at Janelia Farm Research Campus have developed one-photon and ...
Whole-brain imaging with light-sheet fluorescence microscopy and optically cleared tissue is a new, rapidly developing research field. Whereas successful attempts to clear and image mouse brain have been reported, a similar result for rats has proven difficult to achieve. Herein, we report on creati...
Dr. Jan Huisken, Director of Medical Engineering at the Morgridge Institute for Research has been instrumental in the invention and development of Light Sheet Microscopy. One of his current research interests is in the development of novel methods to image cleared tissues. Dr. Kurt Weiss, postdocto...