Efficient Knockin Mouse Generation by ssDNA Oligonucleotides and Zinc-Finger Nuclease Assisted Homologous Recombination in Zygotes.doi:10.1371/journal.pone.0077696Research ArticleThe generation of specific mutant animal models is critical for functional analysis of human genes. The conventional gene targeting ...
We offer knockin mouse model service from strategy design to F1 mice. ESC Gene knockin services include conditional knockin, humanized & Rosa26 knockin.
Progranulin inhibits fibrosis by interacting with and up-regulating DNAJC3 during mouse skin wound healing Cellular SignallingDOI:10.1016/j.cellsig.2023.110770(2023) Testimonials "We generated a mouse knockout model using Cyagen’s TurboKnockout® technology. Cyagen’s team was on time... ...
4.HPRT/Rosa26 Knockin Mouse Screening * Chimera mouse generation and screening Our customer service representatives are available 24 hours a day, from Monday to Sunday. * For research use only. Not intended for any clinical use. Company Address...
-Nuclease injection to obtain founders: The nuclease mRNA and donor vector will be co-injected into fertilized mouse eggs, followed by implantation of the eggs into surrogate mothers to obtain offspring. In cases where the nuclease expression vectors are designed and constructed by Cyagen, we will...
We provide a full catalog of expert model generation services with guaranteed genotype and multiple import options that mitigate quarantine. » Request a Quote or Information Humanized knockin mice - mouse models with an endogenous gene replaced by a human gene or genomic sequence - are used in ...
Generation of a Novel MOP-R Knockin Mouse with Altered Trafficking Properties To directly examine whether endocytosis of the MOP-R in response to morphine alters the development of tolerance and dependence in vivo, we generated a knockin mouse expressing the rMOP-R mutant receptor that internalizes...
Generation of a Cre-Dependent Cas9 Knockin Transgenic Mouse We generated a Cre-dependent Cas9 mouse by inserting a Cas9 transgene expression cassette into the Rosa26 locus (Figure 1A). The transgene consists of a 3× FLAG-tagged Streptococcus pyogenes Cas9 linked via a self-cleaving P2A peptide...
Enhanced sensitivity to N-methyl-D-aspartate receptor activation in transgenic and knockin mouse models of Huntington's disease Molecular pathogenesis of pseudohypoaldosteronism type II: generation and analysis of a Wnk4(D561A/+) knockin mouse model....
Brefeldin A and L755507 increase HDR twofold to threefold in mouse pluripotent stem cells [47]. In addition, RS-1, which upregulates homologous recombination factor RAD51 [56], increases nuclease-mediated knockin efficiency by twofold to fivefold [49, 50]. In contrast to these reports, none ...