本试剂盒使用反转录聚合酶链反应(RT-PCR)检测法,设计特异性的靶标位点,进行 K562 feeder cell 残留检测。 Cat.No. HG-KF001 检测范围:10 ~3.00×106copies/μL 最低检测限度:0.05% 产品特点 · 采用TaqMan 荧光探针原理,专一性强,灵敏度高 ·操作便捷,可实现一小时出结果 ...
再刺激促进NK细胞的恢复和随后的扩增,从而实现临床规模细胞的生产。 NK Cell Expansion Kit BPS Bioscience的NK细胞扩增试剂盒包含生长阻滞的NK饲养层细胞和NK细胞基础培养基。其中NK饲养层细胞是经改造的K562细胞,可支持纯化NK细胞或外周血单个核细胞(PBMC)的扩增。 图1.扩增过程中的 NK细胞流式检测 图2. NK细胞...
Commonly used feeder cell lines are RPMI8866, Epstein-Barr lymphoblastoid cell line (EBV-LCL), and K562. Stimulation with NK-sensitive K562 cells is known to augment NK cell proliferation to IL-2, IL-15, and IL-21 in combination.Recently, remarkable NK cell-expansion rates are achieved when...
Commonly used feeder cell lines are RPMI8866, Epstein-Barr lymphoblastoid cell line (EBV-LCL), and K562. Stimulation with NK-sensitive K562 cells is known to augment NK cell proliferation to IL-2, IL-15, and IL-21 in combination.Recently, remarkable NK cell-expansion rates are achieved when...
A Serum- and Feeder-Free System to Generate CD4 and Regulatory T Cells from Human iPSCs iPSCs can serve as a renewable source of a consistent edited cell product, overcoming limitations of primary cells. While feeder-free generation of clinica... Fong Helen,Mendel Matthew,Jascur John,... ...
feeder cellinactivationBackground/Aim: gamma-Irradiation has been proven to be the most effective method to inactivate K562 cells, but gamma-irradiators are not available in some institutes. This study was designed to compare the effects of X-ray and gamma-irradiation on K562 cells in natural ...
In order to evaluate the therapeutic efficacy of NK cell adoptive immunotherapy in preclinical models with a potential for clinical translation, there is a need for a reliable platform for ex vivo expansion of NK cells. Numerous methods are reported in literature using cytokines and feeder cells ...
Obtaining the progeny of a single cell by cloning the original population is one of the ways to study NK cell heterogeneity. In this work, we sorted single cells into a plate and stimulated them via interleukin (IL)-2 and gene-modified K562 feeder cells that expressed membrane-bound IL-21...
Further, a recombinant soluble NKG2-C-receptor binds specifically to K562 cells, which are target cells for NK cell killing, and to RPMI 8866 cells, which are feeder cells for NK cells; several other hematopoietic cell lines tested do not show any binding. The binding structures on the ...
Obtaining the progeny of a single cell by cloning the original population is one of the ways to study NK cell heterogeneity. In this work, we sorted single cells into a plate and stimulated them via interleukin (IL)-2 and gene-modified K562 feeder cells that expressed membrane-bound IL-21...