再刺激促进NK细胞的恢复和随后的扩增,从而实现临床规模细胞的生产。 NK Cell Expansion Kit BPS Bioscience的NK细胞扩增试剂盒包含生长阻滞的NK饲养层细胞和NK细胞基础培养基。其中NK饲养层细胞是经改造的K562细胞,可支持纯化NK细胞或外周血单个核细胞(PBMC)的扩增。 图1.扩增过程中的 NK细胞流式检测 图2. NK细胞...
Commonly used feeder cell lines are RPMI8866, Epstein-Barr lymphoblastoid cell line (EBV-LCL), and K562. Stimulation with NK-sensitive K562 cells is known to augment NK cell proliferation to IL-2, IL-15, and IL-21 in combination.Recently, remarkable NK cell-expansion rates are achieved when...
本试剂盒使用反转录聚合酶链反应(RT-PCR)检测法,设计特异性的靶标位点,进行 K562 feeder cell 残留检测。 Cat.No. HG-KF001 检测范围:10 ~3.00×106copies/μL 最低检测限度:0.05% 产品特点 · 采用TaqMan 荧光探针原理,专一性强,灵敏度高 ·操作便捷,可实现一小时出结果 ...
Commonly used feeder cell lines are RPMI8866, Epstein-Barr lymphoblastoid cell line (EBV-LCL), and K562. Stimulation with NK-sensitive K562 cells is known to augment NK cell proliferation to IL-2, IL-15, and IL-21 in combination.Recently, remarkable NK cell-expansion rates are achieved when...
Further, a recombinant soluble NKG2-C-receptor binds specifically to K562 cells, which are target cells for NK cell killing, and to RPMI 8866 cells, which are feeder cells for NK cells; several other hematopoietic cell lines tested do not show any binding. The binding structures on the ...
In order to evaluate the therapeutic efficacy of NK cell adoptive immunotherapy in preclinical models with a potential for clinical translation, there is a need for a reliable platform for ex vivo expansion of NK cells. Numerous methods are reported in literature using cytokines and feeder cells ...
Conclusion: Our results suggest that X-ray treatment can be used as an alternative to gamma-irradiation for K562 cells inactivation in human NK cell expansion.Kim, Gwang HoHoang-Nguyen DangMinh-Trang Thi PhanKweon, Soon HoChun, SejongCho, Duck...
In this work, we sorted single cells into a plate and stimulated them via interleukin (IL)-2 and gene-modified K562 feeder cells that expressed membrane-bound IL-21 (K562-mbIL21), which led to a generation of phenotypically confirmed and functionally active NK cell clones. Next, we applied...
Immunophenotypic features of expanded NK cells (acceptor cells) and K562-antiCD19BBζ cells (feeder cells).FuNan ChoTsungHsien ChangChihWen ShuMingChin KoShuenKuei LiaoKangHsi WuMingSun YuShyhJer LinYingChung HongChienHsun Chen
Keywords: adaptive NK cells;effective expansion;K562-HLA-E feeder cells;long-term persistence;single KIR NKG2C