个体外周mNK细胞杀伤活性的抑制效应结果与转染了空质粒的对照组相比,外周血NK细胞对K562G1的杀伤率降低3243(P<001)结论靶细胞表达HLAG1分子可明显抑制NK细胞的杀伤效应::关键词:HLAG1;NK细胞;转染[中图分类号]R39212[文献标识码]AExpressionofHLA--G1antigenonK562cellinhibitscytotoxicityme--diatedbyNKfromPBM...
南方医科大学学报( JS o u thM e dU n iv )- 12 4 5 ・纂瓣;白血病细胞系K 56 2可以诱导外周血N K 细胞凋亡曹燕1’ 2, 段连宁2, 陆承荣2, 罗渊2, 向培德2, 颜述2, 葛淑静2, 张艳君21河北北方学院研究生部, 河北张家口0 7 50 0 0 ; 2解放军空军总医院中心实验室, 北京10 0 14 2摘要...
3.对裸小鼠NK细胞杀伤的耐受性增强,所接种宿主的免疫力受到抑制,形成肿瘤组织内炎性细胞浸润减少。关键词:白血病细胞;致瘤性;裸小鼠StudyofthebiologicalcharacteristicsofhumanhightumorigenicleukemiacelllineK562——ninnudemiceLUShuqing,XUXiaoping,WANGJianmin,eta1.(DepartmentofHaematology,ChanghaiHospital,theSecondary...
K562/IL-21 cell line细胞株 K562-based aAPCs with membrane-bound IL-21 (mbIL21). mbIL21-expressing aAPCs promoted log-phase NK cell expansion without evidence of senescence for up to 6 weeks of culture. Despite the significant increase in proliferation, mbIL21-expanded NK cells also showed a...
培养时间的延长,NK细胞凋亡率显著增高;在相同培养时间的情况下,随着效靶比降低,NK细胞凋亡率逐渐升高,杀伤活性逐渐减低.结论 红白血病细胞系K562可以诱导活化的NK细胞凋亡,这有可能是肿瘤细胞发生免疫逃逸的机制之一.%Objective To investigate the apoptosis of NK cells induced by the erythroleukemia cell ...
1.The Effect and Molecular Mechanism for Artesunate on the CD34~+ Cell Separated from K562 Cell Line;青蒿琥酯对K562细胞系CD34~+细胞的作用及分子机制 2.Effect of Tiopronin to Reverse the ROM Mediated Inhibition of NK Cell-mediated Killing of K562 Cell in Vitro;硫普罗宁体外逆转ROM对NK细胞抗...
In this chapter, a robust NK cell expansion protocol is described using K562 cell line gene modified to express membrane bound IL21 (K562 mb.IL21). We had previously demonstrated that this platform enables the highest fold expansion of NK cells reported in the literature to date (>47,000-...
A monoclonal antibody (MoAb 11-4) was raised against K562, a human erythroleukemia cell line sensitive to natural killer cell-mediated cytotoxicity (NK-CMC). Immunological analysis revealed MoAb to be IgG 2b. Alone, the MoAb was not cytotoxic for K562 and did not bind to the effector cells...
It is very important to quantitate the cytotoxicity of natural killer cell (NK cell) in the fields of cell immune assessment and life science studies. The currently used methods are based on labelling target with radioactivity materials such as ~(51)CrO_4~(2-), [~3H] thymidine and 关键词...
Commonly used feeder cell lines are RPMI8866, Epstein-Barr lymphoblastoid cell line (EBV-LCL), and K562. Stimulation with NK-sensitive K562 cells is known to augment NK cell proliferation to IL-2, IL-15, and IL-21 in combination.Recently, remarkable NK cell-expansion rates are achieved when...