Thus, K562/Zneo cell line is thought to be useful for the nonradioactive assay of human NK and lymphokine-activated killer activities.doi:10.1016/0022-1759(93)90283-DHitoshi OhmoriHidenori IkedaTakahiro TanigawaToshiyuki TakaiMasaki Hikida
结论:NK细胞与K562细胞持续性接触后,双方发生了相互免疫编辑作用;NK细胞 的杀伤活性下降,其分子 机制是细胞表面相应的配受体发生了变化. [关键词]K562细胞;杀伤细胞,天然;NKG2D;MHCI类链相关分子;免疫编辑 [中图分类号]R392.12[文献标识码]A ReciprocalimmunoeditingbetweenNKcellsandK562cells ...
NK细胞杀伤活性 参考文献 1栾风君,杨纯正,马建国等.一株红白血病多药耐药细 胞系(K562/AO2)的建立及其耐药特性的研究.中华肿瘤杂志, 1993 正文 作者:**转牛新清郭坤元周健魏红梅 摘要:本研究探讨K562亲本细胞株及多药耐药细胞株 K562/AO2细胞表面HLAI类分子和MHCI类链相关分子(MICA/B)的 表达及其对NK细胞杀伤...
NK cells were analyzed by flow cytometry. The cytotoxicity and antibody-de-pendent cell-mediated cytotoxicity (ADCC)of the expanded NK cells were assessed by a Cell Counting Ki-8-based colori-metric assay. Results:In the cell preparations from healthy subjects,ex vivo expansion for 3 weeks by ...
562/A O 2cell li ne s w ere ana l y zed by f l ow cy t om etry .C y t o t o x icity o f NK cells (iso lated from 3hea lt hy person s)ag ainst K 562and K 562/AO 2ce lls w ere detected by LDH releasi ng assay at different e ffec t to targe t cell ...
目的 建立一种准确,稳定的自然杀伤细胞(NK)活性检测方法.方法通过电穿孔法将含增强型绿色荧光蛋白(EGFP)的质粒转入K562细胞,经G418筛选后进行克隆,获得EGFP阳性表达细胞株;采用流式细胞仪和乳酸脱氢酶法检测10名健康人外周血NK细胞活性.结果通过基因转染的方法得到1株稳定表达EGFP的K562细胞,转染EGFP对细胞生长无影响,...
They were pulsed with a NKT ligand, αGalactosylceramide and tested as aAPC for NKTs using CFSE proliferation assay. We found that aAPC clones with an intermediate level of CD1d expression induced the highest rate of NKT-cell proliferation. No difference was observed between K562/CD1d and HLA-...
1 Treichel RS, Olken S. The relationship between MDR and resistance to NK and LAK lysis in human leukemic cell lines. Int J Cancer, 1992,50:305-310. 2 曹建平,初俊杰. 抗CD3单克隆抗体激活的杀伤细胞抗瘤活性的研究. 中国免疫学杂志,1993, 9:351. ...
The T cell were activated to be tumor specific CTL after DCs and exosomes were co-cultured with autologous T cells derived from healthy volunteers′ PBMNC. The effect of CTL on K562 cells was detected by MTT assay. The results showed that treatment of T cells with exosomes derived from ...
福建医科大学 硕士学位论文 反应性氮代谢物及其清除剂影响NK细胞抗K562细胞效应的实验 研究 姓名:*** 申请学位级别:硕士 专业:内科学(血液肿瘤) 指导教师:**新 20080301 ABMT Bcl.2 DD decONOO. DHT DM[EM DMSO ELISA E/T FADD FLICE FBS FITC GSH GST.S IFN.Y IL.2 IL.6 I丑6M ⅪR MO MTT NADP...