NK细胞活化、自然杀伤功能检测(NK Cell Activation and Natural Cell Killing Assay) 自然杀伤细胞(NKs)是先天免疫系统中至关重要的产生细胞毒性的淋巴细胞。NK细胞在脊椎动物适应性免疫应答反应中起着类似于T细胞的细胞毒杀作用。 除了搞清楚自然杀伤细胞在先天
The cytolytic activity of PBL was augmented significantly when the cells were preincubated with interleukin-2 for 20 h. This enzyme release assay showed a comparable sensitivity to that of 51 Cr release assay. Thus, K562/Zneo cell line is thought to be useful for the nonradioactive assay of...
NK Cell Killing Assays Available at Reaction Biology Assay options Study example: ADCC assay performed with PBMCs Study example: ADCC assay performed with isolated NK cells Study example: NK engager molecule testing Reaction Biology offers a versatile suite of immuno-oncology assay setups. Since ever...
Ex vivo innate NK cell activity assay NK cell degranulation activity was determined through expression of cell surface CD107a, as previously described54. Cryo-preserved PBMCs were labeled with anti-NKG2a/c-PE (clone Z199, cat. IM3291U; Beckman Coulter) conjugated with anti-PE MicroBeads (Milt...
k, The ADCC (antibody-dependent cell-mediated cytotoxicity) activity assays of NK cells. Target cells, HepG2 cells (An EpCAM+ liver cancer cell line); Ab, humanized antibodies against EpCAM; peripheral NK cells pre-treated with D609 (25, 100, 400 μM) or PBS (control) for 24 h...
1) NK Cell Activity NK 杀伤活性 2) Natural killer cell activity NK杀伤活性 3) NK cytotoxic index NK细胞杀伤活性 1. In order to determine discuss whether 4 factors,the ratio of effector cells and target cells,time of incubation and color precipitation,and the FCS concentration supplemented in ...
(2002) Flow cytometric cytotoxicity assay for measuring mammalian and avian NK cell activity. Cytometry 47 :158-162.Flow cytometric cytotoxicity assay for measuring mammalian and avian NK cell activity. Vizler Csaba,Nagy Tünde,Kusz Erzsébet,Glavinas Hristos,Duda Erno. Cytometry . 2002...
Allogeneic cell clearance was calculated according to the following formula: allogeneic cell clearance rate = [1 − original ratio of CFSEhigh: CFSElow/experimental ratio of CFSEhigh:CFSElow] × 100 in uninfected recipient mice. In vivo CTL cytotoxicity assay The analysis of in vivo CTL ...
Evaluation of a Flow Cytometry‐Based Assay for Natural Killer Cell Activity in Clinical Settings Natural killer (NK) cells are not only important in first line defence against viral and bacterial infections, but also in immune surveillance of malignant... R Valiathan,JE Lewis,AB Melillo,... ...
dervied from malignant tumors of the urinary tract, and the effects of human interferon-β (IFN) on augmentation of NK cell activity.Peripheral blood lymphocytes (PBL) from the buffy coat, obtained from healthy male volunteers, were separated by centrifugation on a Ficoll-Conray density gradient...