To improve the diagnosis of HLH, we aimed to establish a rapid and reliable NK cell activity assay that avoids the use of radioactivity. The K562 cell line, as standard NK target cells, was engineered to stably express enhanced green fluorescent protein (EGFP), which can be quantified by ...
获取事件过少可能会导致结果存在异常。 参考文献:Lorenzo-Herrero S, Sordo-Bahamonde C, Gonzalez S, López-Soto A. CD107a Degranulation Assay to Evaluate Immune Cell Antitumor Activity. Methods Mol Biol. 2019;1884:119-130. doi:...
NK Cell Killing Assays Available at Reaction Biology Assay options Study example: ADCC assay performed with PBMCs Study example: ADCC assay performed with isolated NK cells Study example: NK engager molecule testing Reaction Biology offers a versatile suite of immuno-oncology assay setups. Since ever...
使用Nikon直立显微镜观察NK细胞介导的细胞溶解效应。加入效应细胞后,当细胞指数(Cell Index)相比对照下降到50%时,取出细胞用80%的甲醇固定5分钟,使用Giemsa染色。镜检细胞形态学并用CCD相机进行记录。 实验数据分析 应用xCELLigence系统自带的软件,可对实验全过程,即从接种到细胞溶解,进行显示。通过实时显示时间-E/T(效...
参考文献:Lorenzo-Herrero S, Sordo-Bahamonde C, Gonzalez S, López-Soto A. CD107a Degranulation Assay to Evaluate Immune Cell Antitumor Activity. Methods Mol Biol. 2019;1884:119-130. doi:10.1007/978-1-4939-8885-3_7 本文来自流式中文网(flowcyto.cn)欢迎转发到朋友圈,但谢绝复制粘贴转载如有需要...
CD107a Degranulation Assay to Evaluate Immune Cell Antitumor Activity. Methods Mol Biol. 2019;1884:119-130. doi:10.1007/978-1-4939-8885-3_7. 6. Hayakawa Y, Huntington ND, Nutt SL, Smyth MJ. Functional subsets of mouse natural killer cells. Immunol Rev. 2006 Dec;214:47-55. doi: 10.111...
(2002) Flow cytometric cytotoxicity assay for measuring mammalian and avian NK cell activity. Cytometry 47 :158-162.Flow cytometric cytotoxicity assay for measuring mammalian and avian NK cell activity. Vizler Csaba,Nagy Tünde,Kusz Erzsébet,Glavinas Hristos,Duda Erno. Cytometry . 2002...
Data represents NK cell cytotoxicity assay at an E:T ratio of 2:1. B Statistical analysis of NK cell cytotoxicity. ****P<0.0001, ***P<0.001, **P<0.01, *P<0.05. 3 讨论 CD39 是腺苷通路的重要分子,能够将 ATP 水解为 ADP 进而被 CD73 分子作用下变成腺苷,在肿瘤微环境 中,大量的腺苷分子...
k, The ADCC (antibody-dependent cell-mediated cytotoxicity) activity assays of NK cells. Target cells, HepG2 cells (An EpCAM+ liver cancer cell line); Ab, humanized antibodies against EpCAM; peripheral NK cells pre-treated with D609 (25, 100, 400 μM) or PBS (control) for 24 h...
Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. The specific activity of Recombinant human IL-15 is ≥ 1.0 × 107IU/mg. which is calibrated against human IL-15 WHO International Standard (NIBSC code: 95/554) Recombinant Human Vitronectin(Cat#GMP-C395) Measured ...