Stimulation with NK-sensitive K562 cells is known to augment NK cell proliferation to IL-2, IL-15, and IL-21 in combination.Recently, remarkable NK cell-expansion rates are achieved when genetically engineered (GE) feeder cells are used. Dr. Dario Campana's group found that membrane-bound ...
Here, we describe an in vitro expansion method of human NK cells among PBMCs by coculturing with GE_K562 cells. 展开 关键词: Natural killer cell Expansion K562 Feeder cells DOI: 10.1007/978-1-4939-3684-7_14 被引量: 2 年份: 2016 ...
Vγ9Vδ2 T cells are a minor subset of lymphocytes in the peripheral blood that has been extensively investigated for their tolerability, safety and anticancer efficacy. A hindrance to the broad application of these cells for adoptive cellular immunotherapy has been attaining clinically appropriate num...
Immunophenotypic features of expanded NK cells (acceptor cells) and K562-antiCD19BBζ cells (feeder cells).FuNan ChoTsungHsien ChangChihWen ShuMingChin KoShuenKuei LiaoKangHsi WuMingSun YuShyhJer LinYingChung HongChienHsun Chen
Further, a recombinant soluble NKG2-C-receptor binds specifically to K562 cells, which are target cells for NK cell killing, and to RPMI 8866 cells, which are feeder cells for NK cells; several other hematopoietic cell lines tested do not show any binding. The binding structures on the ...
Keywords: adaptive NK cells;effective expansion;K562-HLA-E feeder cells;long-term persistence;single KIR NKG2C
One of the first methods was based on the use of limiting the dilution and cultivation of cells in a medium supplemented with irradiated (EBV)-transformed B cells or irradiated allogeneic lymphocytes as feeder cells [9,10]. In other studies, the method of co-cultivation of NK cells with ...