Physiologically, proinflammatory cytokines activate ITCH to maintain BRAF activity and to promote proliferation and invasion of melanoma cells, whereas the ubiquitination-deficient BRAF mutant displays compromised kinase activity and reduced tumorigenicity. Collectively, our study reveals a pivotal role for ...
Mechanistically, YAP physically interacts with NLRP3 and maintains its stability through blocking the association between NLRP3 and the E3 ligase β-TrCP1, the latter promotes the proteasomal degradation of NLRP3 via K27-linked ubiquitination at lys380. Thus, we uncover a role of YAP in ...
K27-linked polyubiquitination. Flag-tagged TRIF or its mutants were individually transfected into HEK293 cells along with HA-ubiquitin (K27O). Twenty hours after transfection, ubiquitination assays were performed with the indicated antibodies.dEffects of mutation of various lysine residues of TRIF on ...
Demethylation of H3K27 Regulates Polycomb Recruitment and H2A Ubiquitination Methylation of histone H3 lysine 27 (H3K27) is a posttranslational modification that is highly correlated with genomic silencing. Here we show that human U... M.,G.,Lee,... - 《Science》 被引量: 1098发表: 2007年...
Further experiments revealed that TUFM needs to undergo ubiquitination modification before being recognized by the macroautophagy/autophagy receptor protein SQSTM1/p62, and E3 ubiquitin ligase RNF185 catalyzes K27-linked polyubiquitination of TUFM through the interaction between RNF185's transmembrane ...
Semi-synthesis of K27-linked-mixed-triubiquitin chains through a combination of enzymatic reaction with CAACU strategy - ScienceDirectAtypical ubiquitin chainsCAACU strategyEnzymatic methodProtein ubiquitinationAtypical ubiquitin (Ub) chains were reported to be involved in certain significant cellular ...
These results indicate that a signaling regulatory mechanism involving CHIP-mediated non-degradative ubiquitination of STX17 is necessary for autophagosome-lysosome fusion.doi:10.1038/s41467-024-53002-0Hyunjin Rhohttps://ror.org/01wjejq96grid.15444.300000 0004 0470 5454Department of Biochemistry, College...
RNF185 catalyzes K27-linked poly-ubiquitination of cGAS.Wang, QiangHuang, LiyuanHong, ZeLv, ZhongshiMao, ZhaominTang, YijunKong, XiufangLi, SenlinCui, YeLiu, Heng
UbiquitinationLysosomal degradationIL-3/STAT5 signaling pathway is crucial for the development and activation of immune cells, contributing to the cellular response to infections and inflammatory stimuli. Dysregulation of the IL-3/STAT5 signaling have been associated with inflammatory and autoimmune ...