K63-linked ubiquitination of CALML5 was found in breast cancer tissue, but not found in surrounding healthy tissue.Our findings support the concept that ubiquitination of CALML5 in the nucleus is involved in the carcinogenesis of breast cancer in premenopausal women....
UBIQUITINATIONGENEexpressionCELLproliferationF-box proteins play pivotal roles in multiple cellular processes; however, little is known about their functions in glioma progression. In this study, we found that expression of the F-box and leucine-rich repeat protein 18 ( FBXL18) is significantly up...
Ubiquitination assayCo-immunoprecipitationWestern blotThe Nuclear factor-kappaB (NF-κB) family of transcription factors plays critical roles in inflammatory responses and host defense; however, uncontrolled NF-κB activation can be deleterious by promoting autoimmune diseases and cancers. Lysine K63 (K63...
disease. The Crohn's disease susceptibility protein, NOD2, helps coordinate cytokine responses upon intracellular exposure to bacteria, and this signal coordination by NOD2 is accomplished, in part, through K63-linked polyubiquitin chains that create binding surfaces for the scaffolding of signaling ...
depend on theirprotein sequence, structure and specific function, but also involvement in higher structures/complexes [12–14]. Short-lived proteins more often contain the sequence elements that may serve as degradation signals, as N-terminal destabilizingamino acidsor well-exposedubiquitinationsites [...
et al. The structure of the CYLD USP domain explains its specificity for Lys63-linked polyubiquitin and reveals a B box module. Mol. Cell 29, 451–464 (2008). CAS PubMed Google Scholar Wertz, I. E. et al. De-ubiquitination and ubiquitin ligase domains of A20 downregulate NF-κB ...
Activation of the E3 ubiquitin ligases Pellino and TRAF6 via phosphorylation and their concomitant K63-linked poly-ubiquitination promotes the recruitment of additional Ser/Thr kinases [24]. At the same time, TRAF6 and Pellino act back on their upstream kinase, IRAK1, to induce the poly-...
Specifically, K63-linked polyubiquitin chains directly interact with the TIM1 (also known as HAVCR1) receptor of host cells, which enhances virus entry in cells as well as in brain tissue in vivo. Recombinant ZIKV mutants that lack ubiquitination are attenuated in human cells and in wild-type...
2022). Enhancing the connection between Akt and HK2 through K63-linked ubiquitination eventually leads to an increase in the phosphorylation of HK2 on Thr473 and mitochondrial localization, which is involved in glycolysis and tumor development (Yu et al. 2019). Thus, these PTMs greatly complicate...
(Singh et al., 2019). Notably, AREL1 assembles K33-, K48-, and K63-linked polyubiquitin chains (Kristariyanto et al., 2015). Moreover, AREL1 regulates the activity of suppressor of cytokine signaling-2 (SOCS2) protein, targeting it for ubiquitination and proteasomal degradation ...