K63-linked ubiquitination of CALML5 was found in breast cancer tissue, but not found in surrounding healthy tissue.Our findings support the concept that ubiquitination of CALML5 in the nucleus is involved in the carcinogenesis of breast cancer in premenopausal women....
Ubiquitination assayCo-immunoprecipitationWestern blotThe Nuclear factor-kappaB (NF-κB) family of transcription factors plays critical roles in inflammatory responses and host defense; however, uncontrolled NF-κB activation can be deleterious by promoting autoimmune diseases and cancers. Lysine K63 (K63...
As expected, we observed the inability of RNF13 mutant (C258A/H260A) to catalyze the K63-linked ubiquitination of TRIM29 (Fig. 9j), or controlling the protein abundance of TRIM29 and STING (Fig. 9k). All in all, our data prove that in the context of NASH, RNF13 facilities the K...
These adaptor proteins activated TRAF6, which induced the K63-linked ubiquitination of Beclin 1 at Lys117 in the BH3 domain (Shi and Kehrl, 2010). The ubiquitination of Beclin 1 provoked its oligomerization and stimulated autophagy. Furthermore, A20, a well-known deubiquitinating enzyme, ...
Consistent with functional studies of MAGEL2, the interacting proteins are involved in biological processes such as Arp2/3 complex-mediated actin nucleation, protein K63-linked ubiquitination, and endosome to Golgi retrograde transport. We designed a strategy to identify physiologically relevant proteins ...
Co-IP analysis showed that scVHL specifically induced K63-linked ubiquitination of scIκBα, but not K27-linked ubiquitination (Figure 3J, K). In addition, scVHL did not regulate other types of scIκBα ubiquitination (data not shown). These findings indicate that scVHL activates the NF-...
The RNF84 (TRAF5) stabilizes the RAR-related orphan receptor-γt (RORγt) via catalyzing K63-linked ubiquitination, thus facilitating the proinflammatory Th17 cell differentiation and IL17A/IL17F expression to aggravate inflammatory responses.116Consistent with this observation, elevated expression of ...
Protein Phosphatase 1 (PP1) is a major serine/threonine phosphatase in eukaryotes, participating in several cellular processes and metabolic pathways. Due to their low substrate specificity, PP1’s catalytic subunits do not exist as free entities but ins
Thus, it would be interesting to see if a pVHL-mediated K63 ubiquitination of α5 integrin or some other protein involved in the pathway is involved in the observed lysosomal degradation of α5 integrin. However, a role for pVHL in ubiquitination of PKCι while associated with α5 integrin-...
The K63-linked ubiquitination and unanchored K63 polyUbn promote CARDs oligomerization, facilitating the binding of RIG-I/MDA5 to MAVS. Then, the recruitment of TRAFs and FADD by MAVS promotes activation of the TBK1/IKK kinases, which then activate the transcription factors IRF3/7 and NF-κB...