2002/153/pl15Page1 INTRODUCTION MATERIALS SDS-PolyacrylamideGelElectrophoresis In-GelKinaseAssay Autoradiography EQUIPMENT RECIPES INSTRUCTIONS SamplePreparation In-GelKinaseAssay RELATEDTECHNIQUES NOTESANDREMARKS REFERENCES In-GelKinaseAssayasaMethodtoIdentify KinaseSubstrates MarieW.Wooten (Published8October2002) ...
aTo determine further if the OsMAPK5 (referring to OsMAPK5a [translate] ato in gel kinase assay using myelin basic protein (MBP) as a [translate] 英语翻译 日语翻译 韩语翻译 德语翻译 法语翻译 俄语翻译 阿拉伯语翻译 西班牙语翻译 葡萄牙语翻译 意大利语翻译 荷兰语翻译 瑞典语翻译 希腊语翻译 51...
This methodology can be adapted for the identification of novel kinase-substrates or kinase-kinases that participate in the regulation of cell signaling. Here, I review the rationale and principles of an in-gel kinase assay. This strategy relies on co-polymerization of a substrate within the gel...
Schmidt-Arras, D., Leclercq, O., Gherardini, P.F., Helmer- Citterich, M., Faigle, W., Loew, D., et al. (2011) Adaptation of a 2D in-gel kinase assay to trace phosphotransferase activities in the human pathogen Leishmania donovani. J Proteomics 74: 1644-1651....
Elucidating kinase-substrate relationships is critical for understanding how phosphorylation affects signal transduction and regulatory cascades. Using the α catalytic subunit of protein kinase CK2 (CK2α) as a paradigm, we developed an in-gel method to
[translate] aImmunoprecipitation and in Gel Kinase Activity Assay 免疫沉淀反应和在胶凝体激酶活动分析用试样 [translate] 英语翻译 日语翻译 韩语翻译 德语翻译 法语翻译 俄语翻译 阿拉伯语翻译 西班牙语翻译 葡萄牙语翻译 意大利语翻译 荷兰语翻译 瑞典语翻译 希腊语翻译 51La ...
performance of digestion, separation, and analysis allowed one to perform the whole assay rapidly (e.g., only 8 min for digestion or 12.5 min for LC-MS/MS), achieving high reproducibility (coefficient of variation – CV < 8%). Further, the fast on-line digestion also reduced degradation ...
The cholesterol content was analyzed using the Amplex™ Red Cholesterol Assay Kit (see Supplementary Data Set 3). The method of determination differs from the lipidomics used for the rest of the mammalian lipids, therefore the cholesterol results are not presented as the percentage of total lipid...
For example, we have shown that the E3 ubiquitin ligase Mule, the tumour suppressor protein ARF and the deubiquitylation enzyme USP47 modulate DNA repair by controlling cellular levels of DNA polymerase β, and also that polynucleotide kinase phosphatase levels are controlled by ATM-dependant ...
a以及多达数百个控制点 As well as reaches several hundred control points [translate] aImmunoblot analysis showed that the OsMAPK5 protein was [translate] a(Figure 4B). The immunocomplex in gel kinase assay also [translate] 英语翻译 日语翻译 韩语翻译 德语翻译 法语翻译 俄语翻译 阿拉伯语翻译 ...