Boge, A., et al., " A Nonradioactive Assay for the Insulin Receptor Tyrosine Kinase: Use in Monitoring Receptor Kinase Activity After Activation of Overexpressed Protein Kinase C Alpha and High Glucose Treatment, " Anal. Biochem. 231(2):323-32, Nov. 1, 1995....
Due to the inefficient in vitro transcription, the detection of the RNA involving purification and gel electrophoresis is laborious and not always quantitative. Results Herein, we describe a new, non-radioactive, robust and reproducible eukaryotic in vitro transcription assay that has been established ...
Use of a mixture of proteinase-free collagenases for the specific assay of radioactive collagen in the presence of other proteins. Biochemistry 10, 988–994 (1971). CAS PubMed Google Scholar Birkedal-Hansen, H. Catabolism and turnover of collagens: collagenases. Methods Enzymol. 144, 140–...
The protein concentrations of the supernatants were measured using the BCA Protein Assay Kit (Pierce, USA, #23225). 20 µg of total protein samples were boiled in loading buffer and resolved by electrophoresis in Novex 4–20% Tris–Glycine Mini Gel (Thermo Fisher Scientific, USA, #XP0420...
ns non-significance, MOMP mitochondrial outer membrane permeabilization, mMK mature megakaryocyte, cGAS cyclic GMP-AMP synthase, STING stimulator of interferon genes, Ctrl control, IR ionizing radiation, mtDNA mitochondrial DNA, BM bone marrow, dpi day post IR, TBK1 TANK-binding kinase 1, IRF3 ...
The immuno- precipitated proteins were used in a nonradioactive in vitro kinase assay. A Gst-Elk1 fusion peptide was used as a substrate for the detection of kinase activity in the immunoprecipitates. Following the kinase assay, phosphorylation of the Gst-Elk1 substrate was measured by Western...
mouse intraperitoneal PMT administration retarded the growth of xenografted tumors derived from human ovarian cancer SKOV3 Her2+ cells. Conclusions/Significance We conclude that PMT is usually active in suppression of cell proliferation and tumor growth. Introduction There was a time when most medicinal...
Ribosomal protein S6 kinase NSCLS: Non-small cell lung cancer TC: Thyroid carcinoma WDTC: Well differentiated thyroid carcinoma PDTC: Poorly differentiated thyroid carcinoma ATC: Anaplastic thyroid carcinoma PTC: Papillary thyroid carcinoma FTC: Follicular thyroid carcinoma RAI: Radioactive iod...
A Nonradioactive DEHAL Assay for Testing Substrates, Inhibitors, and Monitoring Endogenous Activity. Endocrinology 2016, 157, 4516–4525. [Google Scholar] [CrossRef] Paul Friedman, K.; Watt, E.D.; Hornung, M.W.; Hedge, J.M.; Judson, R.S.; Crofton, K.M.; Houck, K.A.; Simmons,...
DNA strand breaks within single cells were examined by comet assay. Assays were performed under alkaline pH conditions using the Comet Assay Single cell gel electrophoresis Kit, as per the manufacturer’s instructions. Rlip-depleted and non-depleted cells were treated with vehicle (ethanol) or with...