aTo determine further if the OsMAPK5 (referring to OsMAPK5a [translate] ato in gel kinase assay using myelin basic protein (MBP) as a [translate] 英语翻译 日语翻译 韩语翻译 德语翻译 法语翻译 俄语翻译 阿拉伯语翻译 西班牙语翻译 葡萄牙语翻译 意大利语翻译 荷兰语翻译 瑞典语翻译 希腊语翻译 51...
2002/153/pl15Page1 INTRODUCTION MATERIALS SDS-PolyacrylamideGelElectrophoresis In-GelKinaseAssay Autoradiography EQUIPMENT RECIPES INSTRUCTIONS SamplePreparation In-GelKinaseAssay RELATEDTECHNIQUES NOTESANDREMARKS REFERENCES In-GelKinaseAssayasaMethodtoIdentify KinaseSubstrates MarieW.Wooten (Published8October2002) ...
[translate] aImmunoprecipitation and in Gel Kinase Activity Assay 免疫沉淀反应和在胶凝体激酶活动分析用试样 [translate] 英语翻译 日语翻译 韩语翻译 德语翻译 法语翻译 俄语翻译 阿拉伯语翻译 西班牙语翻译 葡萄牙语翻译 意大利语翻译 荷兰语翻译 瑞典语翻译 希腊语翻译 51La ...
In-Gel Kinase Assay as a Method to Identify Kinase Substrates Phosphorylation of proteins by kinases is central to many cellular processes, including signal transduction. Thus, assays to identify or characterize kinases are a key tool for research in this area. Kinase substrates can be incorporated...
Elucidating kinase-substrate relationships is critical for understanding how phosphorylation affects signal transduction and regulatory cascades. Using the α catalytic subunit of protein kinase CK2 (CK2α) as a paradigm, we developed an in-gel method to
We then measured ABA-induced SnRK2.2/2.3/2.6 activation in these mutants by in-gel kinase assay. The ABA-induced SnRK2.2/2.3/2.6 activation was almost completely abolished in theOK100-octandOK100-nonumutants, which resembles that in thesnrk2-tripleandpyl112458mutants (Fig.4f). Interestingly,...
Abstract Terms to be familiar with before you start to solve the test: signal transduction, growth factor signalling, nerve growth factor (NGF), epidermal growth factor (EGF), mitogen‐activated protein kinases (MAPKs), extracellular signal‐regulated kinases (ERKs), MAPK/ERK kinase (MEK), SDS...
in-gel digestionof the embedded cells needs to begin after thegelationof the agarose has been completed. Because the activity of trypsin is strictly dependent on pH, it is kept inactive at pH4.7 until the gelation has completed and is activated by immersing the gel in cell-lysis reagents at...
a以及多达数百个控制点 As well as reaches several hundred control points [translate] aImmunoblot analysis showed that the OsMAPK5 protein was [translate] a(Figure 4B). The immunocomplex in gel kinase assay also [translate] 英语翻译 日语翻译 韩语翻译 德语翻译 法语翻译 俄语翻译 阿拉伯语翻译 ...
proteins extracted from ABA- or mannitol-treated protonemata of wild-type andPpsnrk2disruptants were subjected to the in-gel kinase assay (Figs.1a, b). A decrease of kinase activity correlated with the number of disrupted genes, QKO plants showing little kinase activity after treatments. These...