The assay is conducted during the hold mode. A wide range of assays can be conducted on the components in the gel, without the need for a blotting step. Following completion of the assay, the hold mode is removed and, if desired, the sample components are further separated in the gel....
Real time in-gel assay 优质文献 相似文献 参考文献 引证文献Establishment of duplex LUX real-time fluorescent PCR assay for detecting bovine herpesvirus 1 A duplex LUX fluorescent PCR assay was established for detecting gE and gC gene of bovine herpesvirus type 1(BHV-1) using LUX self-quenched pr...
The reverse in-gel kinase assay to profile physiological kinase substrates. Nat Methods, 4(11) :957 - 962, 2007.X. Li, B. Guan, M.K. Srivastava, A. Padmanabhan, B.S. Hampton, C.J. Bieberich, The reverse in-gel kinase assay to profile physiological kinase substrates, Nat. Methods ...
assay to evaluate kinase activity in the context of proliferation, differentiation, and survival pathways. Introduction The in-gel kinase assay is a powerful method whereby one can directly assess the substrate potential and characterize the phosphorylation of a known or unknown target. The assay relie...
An assay to detect specific plaque-forming cells (PFC) to Vi antigen (Vi) was developed and the optimal conditions for sensitization of sheep erythrocytes (SE) and plaque development were determined. Using PFC and passive hemagglutination (PHA) assays, Vi-specific immune responses of athymic (nude...
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1. Diffusion-in-gel enzyme-linked immunosorbent assay (-ELISA) was standardized and evaluated for the diagnosis of , in comparison with the conventional serological tests indirect immunofluorescence (IFI), passive hemagglutination () and complement fixation (CF). 2. A total of 236 serum samples pos...
aTo determine further if the OsMAPK5 (referring to OsMAPK5a[translate] ato in gel kinase assay using myelin basic protein (MBP) as a[translate]
(A) 0.50 – 0.55 μg of protein bands of ultrapure BSA protein (band Nr. 1-6) were measured using in-gel ninhydrin assay. (B) Absorbance at 570 nm with standard deviation, arithmetic mean, coefficient of variation (CV) and extraction efficiency were calibrated from five independent...
aImmunoprecipitation and in Gel Kinase Activity Assay 免疫沉淀反应和在胶凝体激酶活动分析用试样[translate]