Here we used transcription factor c-Jun to demonstrate optimization of a fluorescent gel shift assay using IVE proteins. The parameters optimized include fluorophore, oligo concentration, cell-free expression system selection and binding buffer conditions. Fluorescence and IVE give a rapid, convenient ...
Eliminating the media removal step from the workflow can result in lower variability and higher Z´ values compared with the standard fluo-4 assay, while also providing an easier and faster assay. Contributions to baseline fluoresc...
Supernatants were quantified using Bio-rad DC protein assay. Equal amounts of proteins were loaded and separated using 10% polyacrylamide gel. Proteins were transferred onto the activated PVDF membrane (Immobilon-FL Merck Millipore) and later blocked using Odyssey blocking buffer. Primary antibodies BT...
Fluorescence detection multiplexing achieves more data from your sample with iBright. Multiplexing can capture up to four proteins in a single blot for more meaningful and representative experiments, a must for western blot publication. For today’s top journals, total protein normalization and high-qu...
6a, b). We examined the localization of UgsL in floral tissues by immunofluorescence assay. Green fluorescence signals for UgsL were present in infected hyphae and stamen filament cells at 6 dpi with U. virens (Supplementary Fig. 6c), suggesting that UgsL can be secreted into rice cells....
Immunoprecipitation, Western blotting, and the native gel electrophoresis were performed following standard protocols (22, 23). Fluorescence Resonance Energy Transfer (FRET) Analysis FRET was performed as described previously (9, 24). Briefly, S2 cells were transfected with the indicated constructs. ...
D Magnetically sorted CSC and non-CSC populations were subsequently FACS sorted into lipid-high and lipid-low populations based on BODIPY fluorescence (top and bottom 20%). Sphere-forming capability was determined by limiting-dilution assay. Non-CSCs showed uniformly low sphere-forming capability. ...
Kit for the detection and quantification of apoptosis at the single-cell level, based on labeling of DNA strand breaks (TUNEL technology); analysis by fluorescence microscopy or flow cytometry. Application: Qualitative detection of apoptosis at the single-cell level by fluorescence microscopy and quant...
not performed blind to the conditions of the experiments. Tecan i-control plate reader software (version 2.0.10.0) was used for measurement and collection of fluorescence, absorbance and luminescence data. Instron Bluehill software (version 3.11.1209) was used for collection of mechanical testing ...
SYBR Safe DNA Gel Stain—Note 8.1 Through intensive research efforts in both chemical synthesis and bioassay development, we have developed rapid and exceptionally sensitive fluorescence-based assays for quantitation of nucleic acids in solutio...