Detection and quantification of C-terminally tagged proteins by in-gel fluorescenceThe analysis of recombinant proteins in complex solutions is often accomplished with tag-specific antibodies in western blots. Recently, I introduced an antibody-free alternative wherein tagged proteins are visualized ...
In this paper, we perform electrophoresis on native green fluorescence protein, along with the reaction products produced by fluorescence labeling. For short ... DB Craig,NJ Dovichi - 《Analytical Chemistry》 被引量: 127发表: 1998年 Role of terminal complement pathway in the heterologous phase of...
et al. Proteome-wide analysis of protein lipidation using chemical probes: in-gel fluorescence visualization, identification and quantification of N-myristoylation, N- and S-acylation, O-cholesterylation, S-farnesylation and S-geranylgeranylation. Nat Protoc 16, 5083–5122 (2021). https://doi.org/...
Gels were stained with SR (Stain i; imaged using standard fluorescence settings) or with one of 14 different CB stain formulations and imaged using IRF (formulations ii and xii shown here; all other CB formulations shown in supplemental Fig. S1). Data from quantitative image analysis of mouse...
Karthikeyan B, Hariharan S, Sasidharan A, Gayathri V, Arun T, Fakhrabadi AA, Madhumitha C (2019) Optical, vibrational and fluorescence recombination pathway properties of nano SiO2-PVA composite films. Opt Mater 90:139–144. https://doi.org/10.1016/j.optmat.2019.01.063 Article Google Schola...
Fluorescence imaging After visualization, nucleic acid gels are documented for record and analysis of electrophoresis results. If samples are stained with a fluorescent dye, special equipment is requiredto excitethe dye with an appropriate lig...
Comparison of separation modes of high-performance liquid chromatography for the analysis of glycoprotein- and proteoglycan-derived oligosaccharides. Porous graphitised carbon (PGC) has been explored for high-performance liquid chromatography (HPLC) of mono- and di-saccharides released from proteoglycans.....
GeneSys image capture Yes GeneTools image analysis Yes GeneDirectory Option Conversion screen Novaglo UV to visible Size Dimensions (h x d x w) cm 62 x 37.5 x 40Categories Gel Documentation System, Hot Products, Instruments Tags Fluorescence Imaging, Gel Doc, Gel Documentation System, Gel Im...
that do not change in expression are seen as spots with a fixed ratio of fluorescent signals, whereas that differ between the samples have different fluorescence ratios. The fluorescent dyes used in DIGE are flours and matched in respect of molecular weight. Two different dye chemistries are ...
C. R. analysis by capillary electrophoresis and laser-induced fluorescence. Nature 368, 269–271 (1994). Article ADS Google Scholar Thormann, W., Lurie, I. S., McCord, B., Marti, U., Cenni, B. & Malik, N. Advances of capillary electrophoresis in clinical and forensic analysis (...