(2007) In-gel fluorescence probing of RNA-RNA interactions. Anal. Biochem. 362, 278-280.Dayie, K.T.; Gumbs, O.H.; Eldho, N.V.; Seetharaman, M.; Thompson, M. In-gel fluorescence probing of RNA-RNA interactions. Anal. Biochem. 2007, 362, 278-280....
et al. A fluorescence-based assay for N-myristoyltransferase activity. Anal. Biochem. 421, 342–344 (2012). Article CAS PubMed Google Scholar Lanyon-Hogg, T. et al. Click chemistry armed enzyme-linked immunosorbent assay to measure palmitoylation by hedgehog acyltransferase. Anal. Biochem. 490...
chemiluminescence.Beginning with 10 ng/lane (far left), two-fold serial dilutions of purified Transferrin were separated by electrophoresis on duplicate gels. In-Gel Westerns were detected with infrared fluorescence (top) and chemiluminescence on film (bottom). Odyssey detection outperformed ...
Intrinsic fluorescence spectra also showed an increase in the apparent transition temperature (T (1/2)) of hBBCK from 43.0 °C to 46.5 °C, 47.7 °C, and 49.9 °C in 0, 0.6, 0.8, and 1.2 M trehalose, respectively. In addition, trehalose significantly blocked the aggregation of hBBCK ...
Notably, several of the Coomassie stain formulations provided infrared fluorescence detection sensitivity to <1 ng of protein in-gel, slightly exceeding the performance of Sypro Ruby. The linear dynamic range of Coomassie Blue infrared fluorescence detection was found to significantly exceed that of ...
The biodistribution of curcumin following the intranasal application of curcumin transferosomes loaded in situ gel and the control curcumin entrapped in situ gel preparations to rabbits was monitored, and fluorescence was detected in different tissues after 3 h and 12 h of a...
The intensity of green fluorescence in the C6-SR-SUS-ISG group was higher than that in the Control group. In Fig. 6 (D), the uptake of Control, C6-SR-SUS, and C6-SR-SUS-ISG by HCECs at 0.5, 1, 2, and 4 h was presented. The uptake of Cou6 by HCECs gradually increased with...
II.Fluorescence detection using io... A high-performance liquid chromatographic method was developed for the determination of warfarin, coumatetralyl, bromadiolone, difenacoum and brodifacoum i... K Hunter - 《Journal of Chromatography A》 被引量: 163发表: 1983年 Methods for improved selectivity...
Detection MethodFluorescence Label or DyeSYBR Gold Product TypeNucleic Acid Gel Stain Quantity500 μL Shipping ConditionRoom Temperature Target MoleculeRNA, DNA Unit SizeEach 內容物與存放 • Supplied as a 10,000X concentrate in DMSOStore at -20°C, protected from light in a dessicator.圖表...
as indicated by the increase in the intensity of the green fluorescence. However, when AST or RHEA@Gel was added to cells incubated with H2O2, the intracellular ROS concentrations decreased compared with those in the H2O2group. Additionally, comparable phenomena were observed for HUVECs and HaCaTs...