Always clean the imaging system prior to imaging the gel to minimize any background fluorescence Perform all incubations for staining and washing steps at room temperature on an orbital shaker set at 1 rev...
The present invention relates to a method of scanning an electrophoretic gel after electrophoresis of fluorescence labelled samples, comprising inserting the gel into a separate low fluorescent scanning unit, in the shape of a gel folder or a frame, and scanning the gel inside the gel folder or ...
62,63 The fluorescence emission of the supernatant was monitored at λemission =511 nm after λexcitation at 423 nm using a spectrofluorometer (FS-2 (Scinco, Korea) fitted with a Xe arc lamp of 150 W, 400 voltage, and a scanning range of 570 nm min-1. The mon...
Fluorescence microscopy to study galactomannan structure in frozen sucrose and milk protein solutions Both the LBG and guar promoted the formation of a phase-separated protein region. Carrageenan successfully reduced the extent of protein/galactomannan phase ... HD Goff,D Ferdinando,C Schorsch - 《...
as indicated by the increase in the intensity of the green fluorescence. However, when AST or RHEA@Gel was added to cells incubated with H2O2, the intracellular ROS concentrations decreased compared with those in the H2O2group. Additionally, comparable phenomena were observed for HUVECs and HaCaTs...
Likewise, a significant drop in in vivo internal pH was measured using EPR (Mäder et al., 1996) and fluorescence imaging (Schadlich et al., 2014) for in situ forming PLGA implants. Development of ISFIs remains challenging, partly due to the lack of suited in vitro release testing ...
The organic structure-directing agent-free synthesis of zeolite Beta was carried out using several zeolite Beta seeds that differed in SiO2/Al2O3 ratio and crystal size. The synthesis was studied using X-ray diffraction, X-ray fluorescence, scanning electron microscopy, transmission electron microscop...
Sodium fluorescence (0.003%, w/w) was added for color and stirred with heat until a clear light-green colored solution was obtained. The agarose solution was coated on glass slides and cooled until it formed a gel to simulate the human gum cavity. A 50 μL aliquot of the prepared ISG ...
at an enzyme loading of 25 g/mol of silane. The characterization of the immobilized biocatalyst and the correlation of its catalytic efficiency with the morphological and physicochemical properties of the sol-gel matrix was accomplished through scanning electron microscopy (SEM), fluorescence microscopy ...
Because cleaves together with the substrate on the sample photoluminescence intensity also exists differently, therefore this experiment uses the RPM2000 rapid scanning light fluorescence spectrometer, uses in by the mapping method selection surface luminous intensity even consistent sample cleaning[translate]...