FLUORESCENCEPROTEINSSIGNAL-to-noise ratioWESTERN immunoblottingUBIQUITIN ligasesRecombinant proteins in complex solutions are typically detected with tag-specific antibodies in Western blots. Here we describe an antibody-free alternative in which tagged proteins are detected directly in polyacrylamide gels. For...
Figure 9.2.7 Quantitative analysis of six different proteins using the NanoOrange Protein Quantitation Kit (N6666): A) bovine serum albumin (BSA, ), trypsin () and carbonic anhydrase (); B) IgG (), streptavidin () and RN...
The goal of skin resurfacing is the rejuvenation of the skin by stimulation of its regenerative potential. The outer layer of epidermis is ablated in order to improve the quality of the skin. Sun damage is removed and age-related changes are improved in skin resurfacing. In general, there ar...
Figure 2. Quantitative analysis using the No-Stain Protein Labeling Reagent and an iBright Imaging System.A Novex 4-12% Tris-Glycine gel, WedgeWell format, was loaded and electrophoresed with lysates from HeLa cells expressing RB1, at total protein loads ranging from 0.6 to 10 µg. Protein...
C Model predictions are compared to in vivo fluorescence measurements for these promoters when specifying their upstream and downstream sequences (R2 = 0.79, Spearman’s ρ = 0.85). Data are provided in Supplementary Data 1. Full size image Forward engineering promoters Next, we developed...
5e). Notably, there were no repetitive patterns of fluorescence changes before ultrasound onsets. The applied ultrasound parameters did induce some degree of Ca2+ responses in the Saline+ mice, but the average amplitude in the PGVs+US group was significantly higher under all applied US intensities...
The number of attached cells between each DMECM and SIS coated groups was compared by fluorescence intensity (Ex 494 nm, Em 520 nm) using a microplate reader (TECAN). 2.11. Chemotactic migration assay for hMFCs Quantitative migration analysis was performed using by Boyden Chamber Assay Kit (QC...
For example, developing biomimetic gel-based systems can help to accurately investigate drug release and predict long-term performance of implants in vitro [117,118]. To explore the in vivo process of PLGA implants, radio labeling and fluorescence labeling may be feasible monitoring strategies, ...
Infertility affects one in six couples worldwide, and fertility continues to deteriorate globally, partly owing to a decline in semen quality. Sperm analysis has a central role in diagnosing and treating male factor infertility. Many emerging techniques,
First,Spi1(also known asPU.1) andGata1KO simulation is used to illustrate the CellOracle in silico perturbation analysis. The TF perturbation simulation is visualized as a vector map on the 2D trajectory space (Fig.1dand Supplementary Video1), representing a potential shift in cell identity in...