example, kinase-kinase activation pathways depend on upstream elements, such as Ras, Src, or particular second messengers, which in turn are influenced by genetic elements that can vary. Thus, in-gel kinase assays are a powerful method that can be used in combi- ...
23. To confirm the identity of the kinase activity, proteins extracted from ABA- or mannitol-treated protonemata of wild-type andPpsnrk2disruptants were subjected to the in-gel kinase assay (Figs.1a, b). A decrease of kinase activity correlated with the number of disrupted...
technology in kinase assaysdoi:10.1517/17460440802518171JiaYongExpert Opinion on Drug Discovery
Treatment with DM for 3 days induced the expression of myosin heavy chain (MHC), MyoD, and muscle creatinine kinase (MCK). These increases were attenuated when KDM2B was overexpressed. The overexpression of KDM2B was performed through the transfection of pCMV-3xFlag-KDM2B. The empty vector ...
(LDH-A),aldolase Aand C,pyruvate kinaseM2, andthymidine phosphorylase. TheWarburg effectin RCC is demonstrated with the identification of increases in proteins involved in the majority of steps in the glycolytic pathway and decreases in the gluconeogenic reactions, together with a parallel decrease ...
Chromatographic bioanalytical assays for targeted covalent kinase inhibitors and their metabolites. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 2021, 1162, 122466. [Google Scholar] [CrossRef] [PubMed] Mueller-Schoell, A.; Groenland, S.L.; Scherf-Clavel, O.; van Dyk, M.; Huisi...
Deep learning enables rapid identification of potent DDR1 kinase inhibitors[github][paper] In this paper from InSilico Medicine, which came out to some fanfare in 2019, an approach called GENTRL (Generative Tensorial Reinforcement Learning) was used to do rapid discovery of small-molecule inhibitors...
5′ ends of the blunted DNA fragments are phosphorylated (for efficient subsequent ligation) by an enzyme such asT4 polynucleotide kinase 3′ ends of the blunted DNA fragments are adenylated (A tailing), which is required for ...
In silico identification of viral anti-CRISPR proteins (Acrs) has relied largely on the guilt-by-association method using known Acrs or anti-CRISPR associated proteins (Acas) as the bait. However, the low number and limited spread of the characterized ar
Special thanks to Steve Stippec for his assistance with PKA kinase assays. We thank Dr. Jenna Jewell for providing us with a PKA KO cell line. We also thank Mrs. Sophie Guo with her assistance with Western blot analysis, Dr. Amirmanssor Hakimi for help with DAVID analyses, and Dr. ...