使用Index 1 测序引物混合物进行第一次 Index 读取 (Index 1 / i7,负链作为模板) • Truseq Dual Index Library • Nextera Dual Index Library //步骤 3A (MiSeq, HiSeq 2000/2500, NovaSeq 6000 (v1.0 reagents)): 第二次 Index 读取 (Index 2 / i5,负链作为模板) • Truseq Dual Index Li...
使用Index 1 测序引物混合物进行第一次 Index 读取 (Index 1 / i7,负链作为模板) • Truseq Dual Index Library • Nextera Dual Index Library //步骤 3A (MiSeq, HiSeq 2000/2500, NovaSeq 6000 (v1.0 reagents)): 第二次 Index 读取 (Index 2 / i5,负链作为模板) • Truseq Dual Index Li...
测序引物与片段的 P7 区域结合,并测序 DNA nsert 的另一端。 上面展示的单 Index 的文库测序过程,实际上,有的文库还会有两个 Index,分别称为 i5 和 i7。 这个时候,测序的顺序是先测 Read1 和 i7,再测 i5 和 Read 2: 更加详细的过程请参考 Illumina技术手册...
这个index序列是和Nextera一样的。我涂红色部分就是index序列的反向反义序列,每个index序列是不同的8个碱基,这个和Nextera的i7(部分一样,请自己对照)是一样的。 i5 sequence:ATAC-seq的Adaptor1 里没有barcode N/S502:CTCTCTAT N/S503:TATCCTCT N/S505:GTAAGGAG N/S506:ACTGCATA N/S507:AAGGAGTA N/...
During PCR, barcodes can be incorporated by use of the NEBNext index primers, thereby enabling multiplexing. This kit includes 96 pre-mixed unique pairs of i5 and i7 index primers, packaged in a single-use 96-well plate with a pierceable foil seal. NEBNext Oligos can be used with NEB...
uDual-indexedlibraries—Addsupto24unique8-baseIndex1(i7)sequencesandupto16unique8-base Index 2 (i5) sequences to generate up to 384 uniquely tagged libraries. Duringindexed sequencing, the index is sequenced in a separate read, calledthe Index Read, wherea new ...
i5 sequence:ATAC-seq的Adaptor1 里没有barcode N/S502 :CTCTCTATN/S503 : TATCCTCTN/S505 : GTAAGGAGN/S506 : ACTGCATAN/S507 : AAGGAGTAN/S508 :CTAAGCCTN/S510 :CGTCTAATN/S511 : TCTCTCCGN/S513 : TCGACTAGN/S515 : TTCTAGCTN/S516 : CCTAGAGTN/S517 : GCGTAAGAN/S518 :CTATTAAGN/S520...
u Dual-indexedlibraries—Addsupto24unique8-baseIndex1(i7)sequencesandupto16unique8-base Index2(i5)sequencestogenerateupto384uniquelytaggedlibraries. Duringindexedsequencing,theindexissequencedinaseparateread,calledtheIndexRead,whereanew sequencingprimerisannealed.Whenlibrariesaredual-indexed,thesequencingrun...
uSingle-Single-indexedindexed librarieslibrariesAdds upto48unique 6-base Index 1 (i7)seque 10、nces togenerate up to48 uniquelytagged libraries. uDual-Dual-indexedindexed librarieslibrariesAdds up to24unique 8-base Index 1(i7) sequences andup to16unique 8-base Index 2(i5) sequences to...
Dual-Indexed Workflow on a Paired-End Flow Cell4 Dual-Indexed Workflow on a Single-Read Flow Cell6 Sequencing Primers for HiSeq Systems8 Revision History9 Technical Assistance11 Document#15057455v03 ILLUMINA PROPRIETARY February2017 For Research Use Only.Not for use in diagnostic procedures.