Illumina 是全球高通量测序技术的领导者,提供基于短读长测序(short-read sequencing)的高精度、高通量测序仪。 主要测序技术Illumina 主要使用边合成边测序(Sequencing by Synthesis, SBS)技术。这种方法通过加入荧光标记的碱基,每次插入一个碱基后,通过激光检测荧光信号,逐步测定DNA序列。 其平台广泛应用于基因组学、转录...
[1] KN Natarajan et al. Comparative analysis of sequencing technologies for single-cell transcriptomics. Genome Biol.2019.https://doi.org/10.1186/s13059-019-1676-5 [2] Kim HM., et al. Comparative analysis of 7 short-read sequencing platforms using the Korean Reference Genome: MGI and Illumi...
6 参考文献 [1] Guo Y, Li J, Li CI, Long J, Samuels DC, Shyr Y. The effect of strand bias in Illumina short-read sequencing data. BMC Genomics. 2012 Nov 24;13:666. doi: 10.1186/1471-2164-13-666. PMID: 23176052; PMCID: PMC3532123....
[1]KN Natarajan et al. Comparative analysis of sequencing technologies for single-cell transcriptomics. Genome Biol.2019. https://doi.org/10.1186/s13059-019-1676-5 [2]Kim HM., et al. Comparative analysis of 7 short-read sequencing platforms using the Korean Reference Genome: MGI and Illumina...
转录组测序(RNA-seq)分析根据其是否依赖参考基因组信息,主要分为两大部分:转录组从头测序(de novosequencing)测序及重测序(re-sequencing)。转录组从头测序是在不依赖物种基因组序列信息的前提下,用新一代高通量测序技术对某物种的特定组织或者细胞的转录本进行测序并获取相应的转录本信息的过程。转录组重测序针对的...
Yang, RNA-seq analysis and gene discovery of Andrias davidianus using Illumina short read sequencing, PLoS One 10 (2015), e0123730.F. Li, L. Wang, Q. Lan, H. Yang, Y. Li, X. Liu, Z. Yang, RNA-Seq analysis and gene discovery of Andrias davidianus using Illumina short read ...
[1] Illumina to Acquire Pacific Biosciences for Approximately $1.2 Billion, Broadening Access to Long-Read Sequencing and Accelerating Scientific Discovery. Retrieved November 1, 2018, from https://www.businesswire.com/news/home/20181101006...
Illumina,TG-112-1001,TG TruSeq® Dual Index Sequencing Primer Kit, Single-Read Illumina,WG-15-306,Infinium Accessory Starter Kit - Mid Throughput (110V) Illumina,15017552S,SPARE, CRATE, TECAN 150 SERIES Illumina,11257389S,SPARE,ASSY.TOWER BOARD PCE4-03 Illumina,TG-202-1001,TG TruSeq® ...
Explore the history of Solexa and the sequencing by synthesis (SBS) technology that powers Illumina sequencing instruments.
The short read length makes it challenging to assemble the complete genomes of complex organisms or accurately analyze repetitive regions of the genome. This is where complementary technologies, such as long-read sequencing or optical mapping, come into play. In conclusion, Illumina reads are the ...