HUVEC细胞形态，显微镜(40X)[1]2.完全培养基：可选择基础培养基+血清的培养体系或者成品完全培养基①基础培养基+血清这种完全培养基一般血清的含量是20%，基础培养基的类型可以是TC199（含有多种维生素、无机盐和微量元素等）、M199（含有独特的成分，包括腺嘌呤、腺苷、次黄嘌呤、胸腺嘧啶等）或者RPMI-1640（平衡盐...
you will also find a protocol for HUVEC culture. Our data on HUVEC proteomics are depicted in the 2D gels and Identifications area (identification, number of accession in database, pI and MW) There are also links to databases, softwares for PMF study, technical manuals, journals in the field...
1. Medina-Leyte, D.J., et al., Use of Human Umbilical Vein Endothelial Cells (HUVEC) as a Model to Study Cardiovascular Disease: A Review. Applied Sciences, 2020. 10(3). 2. Marin V, K.G., Grès S, Farnarier C, Bongrand P, Endothelial cell culture: protocol to obtain and cultiv...
Applied Sciences, 2020. 10(3). 2. Marin V, K.G., Grès S, Farnarier C, Bongrand P, Endothelial cell culture: protocol to obtain and cultivate human umbilical endothelial cells. J Immunol Methods, 2001 Aug 1. 254: p. 183-90.
本参考文献提供了使用 Lipofectamine RNAiMAX(货号 13778-075、13778-150)将 Stealth™ RNAi 或 siRNA 转染至人脐静脉内皮细胞(HUVEC 细胞 - ATCC,货号 CRL-1730)中的建议程序。Lipofectamine RNAiMAX 的活性范围较宽,仅需进行少量优化工作即可实现较高敲低水平。
Lipofectamine® LTX 试剂采用专有的无动物源性配方,用于将 DNA 转染到真核细胞中,且具有低细胞毒性。本参考文件提供了使用 Lipofectamine® LTX 试剂(货号 15338-100)将质粒 DNA 转染到 HUVEC 人脐静脉内皮细胞原代分离株(0 代 [Cambrex, East Rutherford,...
tissue culture flasks can usually be established from one vial containing >5 x 105 HUVEC. The procedure given below is a sample protocol for establishing cultures from the contents of one vial.1. Prepare a bottle of supplemented Medium 200 (Cat.no. M-200-500) or Medium 200PRF (Cat. no....
In the last few decades, human umbilical vein endothelial cells (HUVECs) have come to be regarded as a standard model system by vascular biologists to understand general principles of endothelial cell biology and dysfunction. Here, we describe isolation, culture, as well as validation of HUVECs....
SubculturingEvery 3-4 days at 8000 cells/cm2Volumes used in this protocol are for 75 cm2flasks; proportionally reduce or increase amount of dissociation solutions for culture vessels of other sizes. Remove and discard spent medium. Briefly rinse with 1X PBS (ATCC 30-2200), 1 mL/25 cm2 ...
Visitwww.lifetechnologies.com/transfectionor contact Technical Service for other specialized transfection protocols (including cell-type specific advice on use of PLUS™ Reagent and antibiotics, and a protocol for vector-based RNAi). Lipofectamine...