从snDropSeq和DroNc-seq我们提取了足细胞、内皮细胞、系膜细胞共650个单核,通过随机森林算法在肾小球单细胞图谱(glomerular single-cell atlas)中选取了最相似的650个最匹配的单细胞整合分析,所有细胞可以聚类成三种独特的细胞类型,通过MetaNeighbor方法来预测scDropSeq得到的细胞亚群。尽管一些基因的表达数据有所差别,但...
human cell atlasliver cancersingle cell RNAseqspatial transcriptomicstumor‐stroma interactionsMalignant cell growth is fueled by interactions between tumor cells and the stromal cells composing the tumor microenvironment. The human liver is a major site of tumors and metastases, but molecular identities ...
Nevertheless, a landscape of the human brain vasculature at the single-cell level across fetal development, adulthood and various vascular-dependent diseases with a focus on the brain vascular endothelium is lacking. Here we created a comprehensive molecular atlas of the human brain vasculature using ...
The development of single-cell and spatial transcriptomics methods was instrumental in the conception of the Human Cell Atlas initiative, which aims to generate an integrated map of all cells across the human body. These technology advances are bringing increasing depth and resolution to maps of huma...
Wesley, B.T., Ross, A.D.B., Muraro, D.et al.Single-cell atlas of human liver development reveals pathways directing hepatic cell fates.Nat Cell Biol24, 1487–1498 (2022). https://doi.org/10.1038/s41556-022-00989-7 Download citation ...
Together, “multi-omic” atlas now provides an open resource to the worldwide research community for further investigations into the evolution of the human brain and identifying novel targets for disease interventions. Reference: “A single-cell multi-omic atlas spanning the adult rhesus ...
HumanCellAtlas计划和实现进程总结 人类细胞图谱计划(Human Cell Atlas program)是一个全球合作项目,旨在构建人类身体内所有细胞的分类和组织信息。该计划的目标是创建一个详尽的细胞图谱,以便更好地理解人类身体的生理和病理过程。自从该计划的提出以来,已经取得了重大进展并取得了令人瞩目的成果。 该计划的实施过程非常...
内皮细胞(Endothelial Cells):标记基因为VWF(von Willebrand因子)。 免疫细胞(Immune Cells):标记基因为CD207(Langerin)。 纤维母细胞(Fibroblasts):标记基因为COL1A1(胶原蛋白1A1)和COL3A1(胶原蛋白3A1)。 黑素细胞(Melanocytes):标记基因为DCT(酪氨酸酶)。
Single-cell and spatial transcriptomics enables probabilistic inference of cell type topography Article Open access 09 October 2020 Author information Authors and Affiliations Human Cell Atlas Inc, Cambridge, MA, USA Jennifer E. Rood, Samantha Wynne, Lucia Robson, Anna Hupalowska, John Randell, Sa...
Here we perform massively parallel single-cell RNA sequencing (scRNA-seq) of human retinas using two independent platforms, and report the first single-cell transcriptomic atlas of the human retina. Using a multi-resolution network-based analysis, we identify all major retinal cell types, and ...