单细胞RNA-seq(single cell RNA-seq, scRNA-seq)是目前用于细胞类型、细胞状态研究的核心工具,很多实验室也同时围绕scRNA-seq技术建立了多种计算方法并且优化了建库方法,不同方法在如何鉴别分子的细胞来源和文库建立方面有所区别。scRNA-seq技术不断革新、升级的同时,随之产生的高分辨、高通量数据也助力发表了多项研究...
HumanCellAtlas/sctools master BranchesTags Code Single Cell Tools Single Cell Tools provides utilities for manipulating sequence data formats suitable for use in distributed systems analyzing large biological datasets. Download and Installation The sctools package provides both command line utilities and ...
2020年《nature》 浙江大学 郭国骥/张丹 Construction of a human cell landscape at single-cell level单细胞分析是了解复杂系统中细胞异质性的有价值工具。然而,目前还没有针对人类的全面单细胞图谱。在这里,…
Single-cell and spatial transcriptomics enables probabilistic inference of cell type topography Article Open access 09 October 2020 Author information Authors and Affiliations Human Cell Atlas Inc, Cambridge, MA, USA Jennifer E. Rood, Samantha Wynne, Lucia Robson, Anna Hupalowska, John Randell, Sa...
Here, we construct a single-cell multi-omics map of human tissues through in-depth characterizations of datasets from five single-cell omics, spatial transcriptomics, and two bulk omics across 125 healthy adult and fetal tissues. We construct its complement web-based platform, the Single Cell ...
In modern biology, the correct identification of cell types is required for the developmental study of tissues and organs and the production of functional cells for cell therapies and disease modeling. For decades, cell types have been defined on the bas
所属专辑:汉靓的医药科技前沿 音频列表 1 【34】行业深度报告:二代基因测序在癌症领域的应用和发展前景 1184 2018-03 2 【33】从人类细胞图谱计划(Human Cell Atlas)谈单细胞基因测序 1097 2018-03 3 【32】从Bioverative谈血友病疗法:短效、长效、基因治疗(下) ...
继续打卡,最近两篇Science背靠背的文章中的一篇:A human cell atlas of fetal gene expression。曹俊越博士参与的一篇400万细胞通量的文章。 首先:取样测序,感觉检测的基因数目不是很多耶,细胞数目是很多,鉴…
Single-cell analysis is a valuable tool for dissecting cellular heterogeneity in complex systems1. However, a comprehensive single-cell atlas has not been achieved for humans. Here we use single-cell mRNA sequencing to determine the cell-type composition of all major human organs and construct a ...
皮肤细胞(Spinous Cells):标记基因为KRT10(角蛋白10)。 颗粒细胞(Granular Cells):标记基因为FLG(角蛋白基因)。 内皮细胞(Endothelial Cells):标记基因为VWF(von Willebrand因子)。 免疫细胞(Immune Cells):标记基因为CD207(Langerin)。 纤维母细胞(Fibroblasts):标记基因为COL1A1(胶原蛋白1A1)和COL3A1(胶原蛋白...