Differences in human a-, 0-and b-globin gene expression in monkey kidney cells . Cell 30, 173-183 .Humphries RK;Ley T;Turner P;.Differences in human alpha-,beta- and delta-globin gene expression in monkey kidney cells.Cell.1982.173-183...
A retroviral vector containing a 4.4-kb Pst I human beta S-globin gene and a neomycin resistance gene was used to infect NIH-3T3 and mouse erythroleukemia cells (MELC). In MELC, human beta-globin mRNA transcripts are transcribed and properly initiated and spliced. In some cases, there is...
Human γ-globin genes silenced independently of other genes in the ,-globin locus. Nature, 1991, 350∶252-254.Dillon N, Grosveld F. 1991. Human gamma-globin genes silenced in- dependently of other genes in the beta-globin locus. Nature 350:252- 254. http://dx.doi.org/10.1038/350252a...
Structure of the human G gamma-A gamma-delta-beta-globin gene locus. Proc Natl Acad Sci U S A. 1979 Oct; 76 (10):4827–4831.Bernards R, Little PF, Annison G, Williamson R, Flavell RA. Structure of the human G gamma-A gamma-delta-beta-globin gene locus. Proc Natl Acad Sci U ...
The β and δ chains of hemoglobins A and Aare very similar in structure and are encoded by genes that are tightly linked, yet in the red cell β chain production is 40 fold greater than production of the δ chain, providing an excellent model for studying the control of gene expression...
The β-haemoglobinopathies, such as sickle cell disease and β-thalassaemia, are caused by mutations in the β-globin (HBB) gene and affect millions of people worldwide. Ex vivo gene correction in patient-derived haematopoietic stem cells followed by aut
Here we describe the synthesis of TFOs designed to target a site in the human beta-globin gene, which is the locus for mutations that underlie the beta-globinopathies, including sickle cell anemia. The target is an uninterrupted polypurine:polypyrimidine sequence, containing four adjacent cytosines...
A developmentally stable chromatin structure in the human beta-globin gene cluster The DNase I-hypersensitive sites in the human embryonic β -globin gene region have been mapped in erythroid-enriched fractions of disaggregated fetal live... WC Forrester,C Thompson,JT Elder,... - 《Proceedings of...
We have studied the expression of a cloned mutant human β -globin gene in tissue culture cells. The gene, which was previously isolated from the chromosomal DNA of an individual with a low level of normal β -globin expression (β+-thalassemia), contains five mutations inside the large inter...
Regulated expression of the human beta-globin gene has been demonstrated in cultured murine erythroleukemia cells and in mice after retrovirus-mediated gene transfer. However, the low titer of recombinant viruses described to date results in relatively inefficient gene transfer, which limits their useful...