Also there was a difference in stability between the depurinating and non-depurinatingstem-loops, which probably is due to the 2011b. Self-catalyzed site-specific depurination of Gresidues mediated by cruciform extrusion in closed circular DNA plasmids. J Bio Chem.S KodaliKodalisreeja Com...
A retroviral vector containing a 4.4-kb Pst I human beta S-globin gene and a neomycin resistance gene was used to infect NIH-3T3 and mouse erythroleukemia cells (MELC). In MELC, human beta-globin mRNA transcripts are transcribed and properly initiated and spliced. In some cases, there is...
We have studied the expression of a cloned mutant human β -globin gene in tissue culture cells. The gene, which was previously isolated from the chromosomal DNA of an individual with a low level of normal β -globin expression (β+-thalassemia), contains five mutations inside the large inter...
The β and δ chains of hemoglobins A and Aare very similar in structure and are encoded by genes that are tightly linked, yet in the red cell β chain production is 40 fold greater than production of the δ chain, providing an excellent model for studying the control of gene expression...
Regulated expression of the human beta-globin gene has been demonstrated in cultured murine erythroleukemia cells and in mice after retrovirus-mediated gene transfer. However, the low titer of recombinant viruses described to date results in relatively inefficient gene transfer, which limits their useful...
The human β-globin locus is a highly complex genetic system that has become a classical model for studying the regulation of eukaryotic gene expression. The locus consists of five functional β-like globin genes, [varepsilon]-γ-γ-δ-β and a powerful upstream regulatory element, the locus ...
expression of the human 尾 -globin gene (12 months), as opposed to 6 of 11 mice reconstituted with cells infected in the presence of IL-3 and IL-... DM Bodine,S Karlsson,AW Nienhuis - 《Proceedings of the National Academy of Sciences of the United States of America》 被引量: 406发表...
A 52 base pair alternating purine-pyrimidine (RY) repeat sequence lies in the 5' upstream region of the human beta-globin gene. The structural transition of a plasmid containing this repeat was analyzed by two-dimensional gel electrophoresis. These conformational studies indicate that the 52 bp RY...
We have investigated the mechanism, structural correlates, and cis-acting elements involved in chromatin opening and gene activation, using the human beta-globin locus as a model. Full transcriptional activity of the human beta-globin locus requires the locus control region (LCR), composed of a se...
we inserted this region into the 3'UTR of the rabbit beta-globin gene that is linked to the cytomegalovirus promoter and stably transfected it into LLC... R Nanbu,P.A. Menoud,Y Nagamine - 《Molecular & Cellular Biology》 被引量: 213发表: 1994年 Features in the 5′ non-coding sequence...