Alpha-globin, humanTranscriptional regulationLCRWe developed a reverse-transcription polymerase chain reaction assay, performed on single isolated cells, to demonstrate the coexpression of human α1- and α2-globin mRNA in induced mouse erythroleukemic cells containing a single human α-globin locus. ...
Isolation and nucleotide sequence of the rabbit globin gene cluster psi zeta-alpha 1-psi alpha. Absence of a pair of alpha-globin genes evolving in concert. A cloned 13.3-kilobase (kb) region of rabbit genomic DNA contains a cluster of alpha-like globin genes arranged 5'-psi zeta-(3.6 kb...
We have examined the molecular structure of the human alpha globin gene complex from individuals with a common form of alpha thalassaemia in which one of the duplicated pair of alpha genes (alpha alpha) has been deleted (-alpha 3-7). Restriction mapping and DNA sequence analysis of the mutant...
The human alpha-globin complex lies at the tip of the short arm of chromosome 16. It comprises three functional globin genes (5'-zeta2-alpha2-alpha1-3'), the expression of which is strictly dependent on a positive regulatory element located 40-kb upstream, HS-40. This DNase I-hypersensit...
Alpha-Hemoglobin Stabilizing Protein (AHSP) binds to ±-hemoglobin (±-Hb) or ±-globin and maintains it in a soluble state until its association with the -Hb chain partner to form Hb tetramers. AHSP specifically recognizes the G and H helices of ±-Hb. To investigate the degree of intera...
Translation of human globin mRNA: globin synthesis in cells containing Hb Leiden. 来自 NCBI 喜欢 0 阅读量: 14 作者:RF Rieder,GW James 摘要: Most structurally abnormal are present in smaller amounts than HbA in the erythrocytes of heterozygous subjects. In the presence of a , alpha and ...
Their red cells contain 10% mouse alpha/human beta s hybrid hemoglobin, which partially corrects the thalassemic phenotype of the homozygous beta-thalassemic animals. Though the red cells do not sickle, other properties of the human beta s gene in these mice indicate the potential for the ...
DNA sequence comparisons of a 1200-base pair (bp) region in 14 human fetal globin genes in seven linked pairs reveal 31 nucleotide substitutions at positio... PA Powers,O Smithies - 《Genetics》 被引量: 128发表: 1986年 A gradient of sequence divergence in the human adult alpha-globin dupli...
Regulatory sequences of the human fetal-t-globin gene were studied by constructing composite 'y/ft globin promoters and comparing their function to that of intact, promoters in human K562 cells. The.8-globin gene with either 1,600 or 127 basepairs of 0 promoter sequence was not expressed aft...
Because of this differ- ence between adult and fetal hemoglobin the diffusion gradient for CO across the placenta can not be equated with the difference in carboxyhemo- globin per cent saturation ( COHb ) . This relationship is of interest when comparisons are made between maternal and fetal ...