Alpha-globin, humanTranscriptional regulationLCRWe developed a reverse-transcription polymerase chain reaction assay, performed on single isolated cells, to demonstrate the coexpression of human α1- and α2-globin mRNA in induced mouse erythroleukemic cells containing a single human α-globin locus. ...
Translation of human globin mRNA: globin synthesis in cells containing Hb Leiden. 来自 NCBI 喜欢 0 阅读量: 15 作者:RF Rieder,GW James 摘要: Most structurally abnormal are present in smaller amounts than HbA in the erythrocytes of heterozygous subjects. In the presence of a , alpha and ...
Human globin messenger RNA (mRNA) prepared from erythroid cells of patients with sickle cell anemia has been translated in Xenopus laevis oocytes. Addition of hemin to the injected mRNA causes total globin synthesis to increase and the ratio of alpha- to betas-globin synthesis (alpha/betas ratio...
In order to determine whether any heterogeneity exists in the human α-globin chain, i.e. whether the products of the duplicated genes are identical, we have determined the total sequence of 14 α-globin chains: seven of these were abnormal, while six were normal chains from the same ...
Mutations of the β-globin RNA activator abrogated PKR activation and profoundly affected mRNA splicing efficiency. PKR depletion abrogated splicing and spliceosome assembly; recom- binant PKR effectively restored splicing. Excision of the first intron of β-globin induces strand displacement within the ...
Physicochemical measurement of the base composition of mRNA-related sequences of the human alpha and beta globin genes. Mol. Biol. Rep. 1980; 6 :179–183.Tuan D, Forget BG. 1980 Physicochemical measurement of the base composition of mRNA-related sequences of the human alpha and beta globin ...
Isolation and nucleotide sequence of the rabbit globin gene cluster psi zeta-alpha 1-psi alpha. Absence of a pair of alpha-globin genes evolving in concert. A cloned 13.3-kilobase (kb) region of rabbit genomic DNA contains a cluster of alpha-like globin genes arranged 5'-psi zeta-(3.6 kb...
The nucleotide sequence of the two 5$^{\prime}$-homology blocks of human $\alpha $-globin gene duplication units were determined. The sequence difference between the two blocks is essentially zero in the 5$^{\prime}$ portions, and increases gradually toward the 3$^{\prime}$ ends until it...
J Clin Invest 91: 1862–1867 Nicholls RD, Fischel-Ghodsian N, Higgs DR (1987) Recombination at the human alpha-globin gene cluster: sequence features and topo- logical constraints. Cell 49: 369–378 Quentin Y (1988) The Alu family developed through successive waves of fixation closely ...
Detection and characterization of a 3′ untranslated region ribonucleoprotein complex associated with human alpha-globin mRNA stability Mol Cell Biol 1995 15: 1769–1777 Article CAS Google Scholar Russell JE, Liebhaber SA . The stability of human beta-globin mRNA is dependent on structural ...