Binding of globin mRNA, ,-globin mRNA segments and RNA homopolymers by immobilized protein of polysomal globin messenger ribonucleoprotein - Gorlach, Hermann, et al. - 1989 () Citation Context ...ymatic activity through removal of the detergent (14, 23, 41, 44). Renaturation of proteins ...
Quantitative measurement of globin mRNA was performed by poly(A) selection onto a manifold plastic support, coated with oligo(dT). The mRNA was reverse transcribed, followed by quantitative analysis using PCR via the 5 nuclease assay. The individuals treated with rHuEpo showed a more distinct ...
It is concluded that, although the translation of ..cap alpha..- and ..beta..-globin mRNA is a major site of inhibition by glucocorticoids, there is a detectable amount of ..cap alpha..- and ..beta..-globin mRNA translation which results in unequal amounts of globin synthesis and an ...
By subtracting the number of nucleotides in the coding and poly(A) regions, the number of non-coding nucleotides in the beta-globin mRNA were calculated to be 261, 69 more than the 193 present in the alpha-globin mRNA. Comparison of size estimates of newly synthesized globin mRNAs containing...
The 5'-terminal structures of murine alpha- and beta-globin mRNA were determined after incubating cells of the erythropoietic spleens of mice with [methyl-3H]methionine. Globin mRNA was obtained from total cellular RNA by oligo(dT)-cellulose chromatography followed by elution of mRNA from ...
Using as a standard the extent of nucleotide substitutions inferred from the hypervariable amino acid residues of fibrinopeptides A and B, which are thought to change largely by neutral evolution, we show that not all silent mutations in globin mRNA are neutral. The divergence of the sequences...
Nature Genetics (2004) β-Globin mRNA in Ferrara β0-thalassaemia BERNARD G. FORGET DIANE G. HILLMAN Nature (1977) Direct demonstration of β-globin mRNA in homozygous Ferrara β0-thalassaemia patients S. Ottolenghi P. Comi F. Conconi Nature (1977)Sections...
5' & 3' untranslated regions of Xenopus Beta-globin mRNA cloned into pGEM4Z. Insert size: 260 bp. Transcription: transcribe with T7 RNA polymerase. Cloning sites: Bgl II, EcoRV, SpeI Xenbase has a nice description of this vector: http://www.xenbase.org/reagents/vectorAction.do?method=dis...
The 5′ noncoding region sequence of rabbit β-globin mRNA has been determined. This region is 53 nucleotides long, not including the A-U-G initiator sequence or m 7Gppp "cap" structure. 32P-labeled DNA complementary to the 5′ noncoding region was synthesized using reverse transcriptase with...
This paper demonstrates that regulation of synthesis of the two globin chains in the rabbit reticulocyte is complex: cells contain about 1.5 times more ribosome-associated α mRNA than β, but ribosomes initiate translation of each α mRNA only about 65% as frequently as a β mRNA. Hence ...