Beta-actin mRNA localization is regulated by signal transduction mechanisms - Latham, Kislauskis, et al. - 1994 () Citation Context ...ddition of serum to starved cells results in rapid actin filament formation (Ridley and Hall, 1992, 1994). Analogously, �-actin mRNA is rapidly re...
08、beta-actin和GAPDH的3'/5'比值 一、大部分的基因都有beta-action基因和GAPDH基因,很多Affymetrix芯片都将它们设为一组观察RNA降解程度的内参基因。mRNA是按照5’至3’的顺序来降解的,通过比较它们3’相对于中间或者3’相对于5’的信号强度,可以很好地指示出试验质量。 二、beta-action基因对应的探针组: AFFX...
Asymmetric localization of β-actin mRNA has been known to play an important role in guiding cell directionality, although how it instituted this control was largely unknown. We hypothesized that β-actin mRNA targeted to developing focal adhesions at the leading edge to reinforce these ...
英文: Real-time RT-PCR analysis of half-life of beta-actin mRNA in human SK-N-SH cell lines中文: 实时荧光定量分析SK-N-SH细胞中β-actin mRNA的半衰期 英文: Preparation of Actin and Fluorescent Actin Analogs from Plant Cells 中文: 植物肌动蛋白的纯化及荧光标记 ...
SMN has been implicated in the axonal transport of β-actin mRNA in both primary and transformed neuronal cell lines, and loss of this function could account, at least in part, for spinal muscular atrophy onset and pathological specificity. Here we have utilised a targeted screen to identify ...
别沉了呀,
It is not known how the ZBP1-RNA complex achieves asymmetric protein sorting by localizing beta-actin mRNA. Here we show that chicken ZBP1 modulates the translation of beta-actin mRNA. ZBP1 associates with the beta-actin transcript in the nucleus and prevents premature translation in the ...
应用一步法荧光标记逆转录-聚合酶联反应(reverse transcriptase-polymerase chain reaction,RT-PCR)技术检测了大鼠死后不同时间间隔脑、肝、脾、肾管家基因磷酸甘油醛脱氢酶(glyceraldehyde phosphate dehydrogenase,GAPDH)及β-肌动蛋白(β-actin)mRNA的降解情况,旨在为PMI推断提供一种新的指标。
别沉了呀,
In an earlier report, evidence was presented that the down-regulation of beta-actin mRNA during myogenesis was controlled by a region 3' to the promoter of the gene. In this paper we report the location of this regulatory sequence, determined by deletion analysis and the use of chimeric genes...