Quantitative measurement of calretinin and -actin mRNA in rat brain micropunches without prior isolation of RNA. Brain Res Mol Brain Res. 1993; 20 :229–239.Strauss K, Jacobowitz D: Quantitative measurement of
It is not known how the ZBP1-RNA complex achieves asymmetric protein sorting by localizing beta-actin mRNA. Here we show that chicken ZBP1 modulates the translation of beta-actin mRNA. ZBP1 associates with the beta-actin transcript in the nucleus and prevents premature translation in the ...
该cDNA全长为1536bp,5’端非编码区84 bp,3’端非编码区321 bp,编码区1131 bp,编码377个氨基酸;在基因组DNA中,该基因被一个内含子分为两段,内含子位于第41和第42个氨基酸之间,长度为1498bp。 虾夷扇贝β-肌动蛋白基因可以被用于作为定量某种虾夷扇贝mRNA的标准,这为继续研究虾夷扇贝其他功能基因,及其分子生物...
Beta-actin can be used as a reference gene to normalize mRNA or protein levels in quantitative studies of expression. Although some evidence suggests variation in tissue types and developing embryos (PMID: 23099774) if expression is stable across conditions, beta-actin is still considered by many ...
一、大部分的基因都有beta-action基因和GAPDH基因,很多Affymetrix芯片都将它们设为一组观察RNA降解程度的内参基因。mRNA是按照5’至3’的顺序来降解的,通过比较它们3’相对于中间或者3’相对于5’的信号强度,可以很好地指示出试验质量。 二、beta-action基因对应的探针组: ...
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中文: 松江鲈β-肌动蛋白基因全长cDNA的克隆和序列分析 更详细... 英文: Real-time RT-PCR analysis of half-life of beta-actin mRNA in human SK-N-SH cell lines 中文: 实时荧光定量分析SK-N-SH细胞中β-actin mRNA的半衰期 更详细...分享到: 赞助...
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Insulin-like growth factor-1 promotes osteogenic differentiation and collagen I alpha 2 synthesis via induction of mRNA-binding protein LARP6 expression. (2017) Dev.Growth.Differ. 59:94-103 WB ; Mouse. PubMed:28211947 [IF=1.482] Ji and Jiang Effect of shRNA-mediated inhibition of Nanog ...