M6A methylation is generally common in protein-coding transcripts and are localized in the 3′UTRs29 and 5′UTR30 regions. Interestingly, the methylation sites of BCL2A1 are located within the CDS21 and 5'UTR regions18. Based on our data, we propose that alterations in the mRNA methylation ...
The study of gene regulation is dominated by a focus on the control of gene activation or increase in the level of expression. Just as critical is the process of gene repression or silencing. Chromatin signatures have identified enhancers, however, genom
This vector contains a beta-globin 3’UTR repeat followed by a polyA tail which has been reported to enhance RNA stability and translational efficiency (Holtkamp et al., 2006). TMG plasmids were linearized with the restriction enzyme NotI (NEB) followed by in vitro transcription (IVT) using ...
mRNA was capped co-transcriptionally using CleanCap AG analog (TriLink BioTechnologies) resulting in a 5’ Cap 1 structure. In vitro transcription reaction was performed as described previously64,66 and mRNAs were purified using RNeasy kit (QIAGEN). Mammalian-optimized UTR sequences (TriLink ...
(~7.5 kb),145or CRISPR-based tools like cytosine base editor or prime editor 2 (>5 kb).146,147Various strategies have emerged to overcome this hurdle by dividing a large transgene into two parts, with each encapsulated in an rAAV capsid. Co-introduction of these segmented transgenes ...
5 Translational Medicine Research Center, Sirius University of Science and Technology, Olimpiyskiy ave. b.1, 354349 Sochi, Russia * Author to whom correspondence should be addressed. Int. J. Mol. Sci.2023,24(24), 17149;https://doi.org/10.3390/ijms242417149 ...