While wild-type mRNA levels are restored by fusion of the HSV1 Tk 5'-UTR to the nonsense mutated beta-globin reading frame, translation of a wildtype reading frame in such a hybrid is precluded. In contrast, the
the 3' UTRs and the coding region of the "-gIobin cDNA. The cycIing programs of the RACE PCR were one cycIe of 94 C for 5 min;30 cycIes of 94 C for 30 s,54 C for 30 s,72 C for 45 s;foIIowed by one cycIe of 72 C for l0 min. 2. 6 Genomic DNA extraction The Iiver...
Additional experiments show that the beta-globin and alpha-globin mRNA 3'UTRs provide equivalent levels of stability to a linked beta-globin mRNA coding region, suggesting a parallel in their functions. However, destabilization of the antiterminated beta-globin mRNA is independent of active ...
The possibility of enhancing heterologous gene expression in mammalian cells by the introduction of an intron in 3' untranslated region (UTR) was investigated. To this end, a fragment of human betaglobin gene with intron 2 and flanked exon regions was introduced into the vector-encoding green ...
We have defined two stage selector elements (SSE and SSE-2), located in the proximal γ-promoter and y-gene 5'UTR which mediate the preferential interaction of the γ-gene with the L CR in the foetal developmental stage. The activity of both elements is dependent upon the binding of ...
The critical importance of nucleolin binding to the stability of β-globin mRNA may relate to a stem-and-loop motif within its 3′UTR that is predicted by mRNA-folding algorithms. This structure contains the nucleolin-binding site on its right half-stem, opposite a putative binding site for ...
A novel 13 bp deletion in the 3ЈUTR of the -globin gene causes -thalassemia in a Turkish patient. Hemoglobin 17:551-555.Basak AN, O¨ zer A, Kirdar B, Akar N: A novel 13 Bp deletion in the 3ЈUTR of the -globin gene causes -thalassemia in a Turkish patient....
We report two novel p-thalassemia (beta-thal) deletions involving the 5' region of the p-globin gene (HBB). The first deletion spans 538 bp and removes the beta-globin promoter, 5' untranslated region (5'UTR) and most of exon 1. This deletion was identified in a 3-year-old Vietnam...
Also, we detected four SNP sites: 5'UTR+20(C-T), Codon2 (CAC-CAT) , IVSII-16(C-G) and IVSII-666(T-C) in β-thalassemia genes. Conclusion: Our results could be useful for developing molecular screening plans and help prenatal diagnosis of beta thalassemia in Azerbaijan , Iran and ...
In this study, we have identified two novel putative mutations (HBB:c.*+108 A>G and HBB:c.*+132 C>T) in the 3′ untranslated region (3′-UTR) of the β-globin gene and describe their clinical implications.T. BilgenO. A. Clark...