Mutational analysis of the mouse $\\beta$-globin poly(A) signal function in COS cells revealed a striking bipartite structure of the downstream element, located downstream of the poly(A) addition site, and one novel cleavage site determinant for cleavage site selection. From analysis of the ...
The downstream region of the mouse beta (major) globin poly(A) signal was mutated and analyzed for function in transfected COS cells. From analysis of unidirectional Bal31 deletions, the 3' boundary of the downstream element was defined as +22 (22 nucleotides downstream from the cleavage site)...
We constructed a viable insertion mutant (ins 5) of polyomavirus which contains, upstream of the L-strand polyadenylation signal, a 94-nt fragment of rabbit beta-globin DNA. Included in this fragment are all of the sequence elements required for efficient cleavage and polyadenylation of rabbit bet...
17. To initially investigate whether this system could also support HR-based gene editing of mouse HSCs by Cas9-AAV6 technology, we tested HR at theRosa26safe harbor locus (Fig.1A) using a previously validated sgRNA18,19pre-complexed with HiFi Cas9 protein11and an AAV6-repair template contai...
(e.g., an amplification product or amplicon). Preferably, the detectable label is selected such that it generates a signal which can be measured and whose intensity is related to (e.g., proportional to) the amount of bound entity. A wide variety of systems for labelling and/or detecting ...
Mutational analysis of the mouse $\\beta$-globin poly(A) signal function in COS cells revealed a striking bipartite structure of the downstream element, lo... Chen,Jingshan 被引量: 0发表: 1994年 Bipartite structure of the downstream element of the mouse beta globin (major) poly(A) signal ...
In a construct where the AATAAA polyadenylation signal was changed to AATACA a high proportion (35%) of transcripts continued to be polyadenylated at the major site. This suggests a surprisingly high degree of flexibility in the precise polyadenylation signal....
These contradictory analyses highlight the difficulty in resolving deep relationships among globin genes, particularly when the time periods between duplication and speciation events are relatively small, the phylogenetic signal at third codon positions is potentially saturated, and non-synonymous sites may ...