For this example, I will use data generated from the BCA protein assay kit to estimate total protein concentrations. To create the standard curve, I have measured the absorbance of 8 standards (25, 125, 250, 500, 750, 1000, 1500 and 2000 μg/mL total protein) and a blank sample (0 ...
One method of reducing this problem is to use affinity purified or cross-absorbed polyclonal antibodies. To increase assay sensitivity, the detection method for an ELISA can be switched from direct to indirect detection using a polyclonal antibody. ELISA samples A variety of samples can be tested ...
One method of reducing this problem is to use affinity purified or cross-absorbed polyclonal antibodies. To increase assay sensitivity, the detection method for an ELISA can be switched from direct to indirect detection using a polyclonal antibody. ELISA samples A variety of samples can be tested ...
However, please also be aware of the number of tests the kit provides and the amount of sample the kit requires to conduct the assay. Boster Bio’s ready-to-use PicokineTM ELISA kits are provided in 96-well format with removable strips, so you can use a portion now and store the ...
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12. Gene apoptosis: tumor protein p53 and B-cell lymphoma 2 13. New methods for predicting chemotherapy response 14. Chemotherapy assay 15. Multigene biomarkers 16. PET assay 17. Use of chemotherapy predictors 18. Prevention of chemoresistance ...
poorly bypassed by direct ligation (30 terminal mispairs; Fig. 2c) triggers use of an alternate (third) path for their resolution, where end structure is more generally altered, or remodelled, by activity of a ssDNA endo/exonuclease. Artemis is a likely candidate for this activity33,34 (...
Analysis: The absorbance at 280 nm was measured for all protein fractions to determine the amount of total protein. Samples were separated via SDS-PAGE and stained with Coomassie® blue and the enzyme activity was measured using a Malachite green phosphate assay. Results and discussion The ...
to both proteins. However, assessing the thermodynamic protein stabilities showed that the melting temperature,Tm, of AlbAS increased significantly upon albicidin binding from 66 to 81 °C (Fig.3b). Binding of albicidin to AlbAL caused a less-pronounced increase inTmfrom 71 to 74 °C ...
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