MSD™ The Immunogenicity Gold Standard Previous NextMSD™ - Meso Scale Discovery MSD-ECL from Meso Scale Discovery is a proven electrochemiluminescence immunoassay platform for large molecule analysis. The ECL technology enables high sensitivity levels combined with broad assay range, making MSD™...
它是由中性粒细胞和活化巨噬细胞产生的神经递质和细胞毒性物质。虽然NO在生物系统中的分子作用是多种多样的,但NO最重要的作用是激活鸟苷酸环化酶。 图片及说明 Fig. Standard Curve of Nitric Oxide Assay. The y-axis is OD540 and the x-axis is NO2- concentration (μM)....
Recipient bovine calves were sub-inoculated with blood from nilgai challenged with either stage of the parasite, and they were monitored for clinical signs of infection and evaluated by a PCR diagnostic assay. Red blood cells (RBCs) from prechallenged nilgai and B. taurus beef cattle were ...
An in vitro scratch-wound healing assay was used to evaluate the effect of treatments on HUVEC migratory ability. Lines were drawn on the back of 6-well plates using a marker pen, and transverse lines were drawn uniformly at distances of 1 cm between the lines. HUVECs were seeded in th...
Method.The Qubit Protein BR Assay and Qubit Protein Assay were performed according to instructions with samples of 1,000 µg/mL of BSA containing commonly used buffers and contaminants. Assays were performed in triplicate, and RFU values were compared ...
Values outside the range of the standard curve are generally non-linear, which can lead to incorrectly extrapolated values. Samples that generate values higher than the highest standard should be (further) diluted and the assay repeated. If samples fall below the range of the assay, the sample...
Appropriate sample dilutions were carried out in order to maintain detection in the linear part of the standard curve, and PRL concentrations were extrapolated from the OD of each sample. To control for reproducibility of the assay, trunk blood of males injected with domperidone was immediately ...
The assay sensitivity was 0.6 mU/l and the recovery of 94%. Paralellism between dose-response curves containing dilutions of a hypothyroid serum pool was found. The development and validation of this kit has allowed its satisfactorial application for diagnostic and clinical research.Rodríguez...
The inhibitory constant IC50 is evaluated using the curve-fitting program. Results are expressed as means±S.E. and consist of at least four independent measurements. Cell Assay The cell viability is analyzed by incubating transfected HEK-293 cells and CHO cells for 1 h with the corresponding ...
e, LDH assay in S. Typhimurium infected inflammatory BMDMs. The mean and SEM (error bars) are derived from three biological replicates (n = 3). Statistical significance (two-tailed t-test) compared to control (Ctrl): *P < 0.05; **P < 0.01; ns, not significant. Source ...