This is particularly true for the protein assay methods that produce non-linear standard curves. Deciding on the number of standards and replicates used to define the standard curve depends upon the degree of non-linearity in the standard curve and the degree of accuracy required. In general, ...
To create a standard curve in Microsoft Excel, two data variables are required. One set of data must be the independent variable, which is the known values (e.g. protein standard concentrations in a BCA assay), and the other is the dependent variable which refers to the measured values (...
[translate] aProtein concentration was determined by the BCA protein assay reagent kit (Pierce), and bovine serum albumin was used as a standard. 正在翻译,请等待...[translate]
The total protein assay kit ( with standard : BCA method )|BCA法) 基本信息 更多信息 中文名称: BCA法) 中文同义词: BCA法);总蛋白定量测试盒(带标准;考马斯亮蓝法);双缩脲法);双缩脲法)总蛋白定量测试盒(带标准 英文名称: The total protein assay kit ( with standard : BCA method ) 英文同义...
A protein measurement then was obtained for each sample using the BCA Protein Assay (ThermoFisher Pierce, Rockford, IL) using the manufacturer's protocol. An aliquot equivalent to 200 μg was removed and reduced with tris(2-carboxyethyl)phosphine (TCEP, 20 mM) and dithiothreitol (DTT, 50 mM...
the volume needed for each assay series. 100 µl of this solution is Note that pipetting of the standard curve samples must be done required per sample, and the radioactivity should be 5,000-6,000 with Hamilton-type syringes that are exclusively dedicated to use cpm/100 µl tritiated ...
该二辛可宁酸 (BCA) 蛋白检测:与非动物蛋白标准品兼容的还原剂产品具体产品描述详情查看G-Biosciences厂家提供的产品说明书。产品形式 该二辛可宁酸 (BCA) 蛋白检测:与非动物蛋白标准品兼容的还原剂产品形式具体详情查看G-Biosciences厂家提供的产品说明书。保存...
sample using the BCA Protein Assay (ThermoFisher Pierce, Rockford, IL) using the manufacturer’s protocol. An aliquot equivalent to 200 μg was removed and reduced with tris(2-carboxyethyl)phosphine (TCEP, 20 mM) and dithiothreitol (DTT, 50 mM) at 60 °C for 30 min, followed...
Micro BCA Protein Assaydoi:DOXPUB 09-0167-SOP概述:描述了一种测定溶液中蛋白质浓度的方法.适用于牛血清白蛋白和抗生物素蛋白.对其他蛋白质的适用性可根据具体情况确定.用于测量稀释蛋白质溶液的浓度,以支持研发,工艺鉴定,清洁验证研究和其他验证.使用抗生物素作为模型蛋白质.包括的章节:目的,范围,责任,参考和...
(e.g., cell lysate). In such cases, the best standard is one that will produce a normal (i.e., average) color response curve with the selected protein assay method and is readily available to any researcher. BSA is such a protein, and the Pierce Albumin Standards are the most ...