Excel Tutorial 1 : Using Microsoft Excel ( 2007 ) to Create a Standard Curve for Protein ConcentrationExcel, Using MicrosoftCurve, Standard
标准蛋白曲线制作（Standard protein curve）The standard curve made by Coomassie brilliant blue method measurement of protein content Standard curve Generally, the content of the substance is measured by spectrophotometry. The standard curve is first made, and then the content of the substance is ...
Wikis>Setting Up an ELISA Standard Curve Theenzyme-linked immunosorbent assay (ELISA)is a sensitive technique for determining the concentration of a protein in a liquid sample. When performing ELISAs, three different data outputs can be generated: ...
Subsequently, replace the medium flowing through the chip with the prepared 1 mg/ml FITC-Dextran solution for perfusion incubation. F.3.2 Drawing the Standard Curve Prepare a standard curve of concentration versus fluorescence intensity by diluting the FITC-Dextran solution in HBSS buffer in a 96...
标准蛋白曲线制作(Standardproteincurve)ThestandardcurvemadebyCoomassiebrilliantbluemethodmeasurementofproteincontentStandardcurveGenerally,thecontentofthesubstanceismeasuredbyspectrophotometry.Thestandardcurveisfirstmade,andthenthecontentofthesubstanceisdeterminedaccordingtothestandardcurve.Therefore,theproductionofstandardcurves...
For greatest accuracy in estimating total protein concentration in unknown samples, it is essential to include a standard curve each time the assay is performed. This is particularly true for the protein assay methods that produce non-linear standard curves. Deciding on the number of standards and...
(ML)0,0.1,0.2,0.3,0.4,0.5,0.6 0.15mol/LNaCl(ML)10.90.80.70.60.50.4 Coomassiebluereagent(ML)5555555 Shakewell,with1tubeasblankcontrol,andcolorat595nm in1H A595nm 1, 2,withA595nmasordinate,standardproteincontentas abscissa(sixpointsfor10ug,20UG,30UG,40UG,50UG,60 UG),drawthestandardcurve...
[translate] aProtein concentration was determined by the BCA protein assay reagent kit (Pierce), and bovine serum albumin was used as a standard. 正在翻译,请等待...[translate]
Objective:To compare the fitting effects of SPSS and Excel for concentration standard curve of serum Hepatitis B Virus Large Surface Protein(HBV-LP).Methods: Enzyme-linked immunosorbent assay(ELISA)was used to measure the absorbance of standard preparation of HBV-LP.Concentration and absorbance of st...
For each protein concentration, the auto-correlation functions of both the free and bound protein signals were recorded. The system was very stable over time and therefore for each of the increasing amounts of protein exactly the same GUVs could be surveyed. Additionally, the ATTO-647 N-DOPE ...