3. Antibody Concentration Your next step should be to determine the optimum working antibody concentration. The rate of binding between antibody and antigen is affected by their relative concentrations in solution (among other variables). Often you will need to tweak the antibody concentration to get...
thus preventing the antibody from binding to its epitope. This is more expensive but also the best way to determine the antibody’s specificity and therefore to get reliable results. In a multicolor Immunofluorescence experiment you must also pay attention to crosstalk between the selected fluorochrom...
After protein extraction is complete, it is important to determine the protein concentration in your sample. A quantitative protein assay such as BCA will yield the most accurate results. It is also recommended to run SDS-PAGE to evaluate the quality of the lysate to ensure that the lysis is...
To pass small-sized kidney stones naturally in urine, certainhome remediescan be helpful such as increased fluid intake, increased water, lemon and citrus juice consumption and low salt, calcium and protein diet. High water concentration does not allow growth of kidney stones and they can slowly ...
Lean Protein: Lean sources of protein like poultry, lean beef, and eggs are rich in amino acids necessary for sperm production. Protein provides the building blocks for cellular growth and repair, including the development of healthy sperm cells. ...
Due to the comparably high resolution within the nucleotide-binding site of actin, we could directly determine the identity of the nucleotide bound to each subunit. Consistent with the early addition of phalloidin during the polymerization reaction, most subunits show clear density for inorganic ...
The regulation of cellular shape and tension by curvatures at various length scales activates specific mechanotransduction pathways that synergistically determine downstream cellular functions. Changes in the balance of cellular forces required to adapt to curved environments can have dramatic consequences, prom...
The position of unfolding curves of oligomeric proteins depends on the protein concentration. The extent of this dependence is analyzed here in, terms of the midpoint concentration, i.e., the denaturant concentration at which the fractions of folded and unfolded protein are equal. Reexamination ...
It’s well known that exercise leads to increased blood flow to the brain, which has several cognitive benefits, such as alertness, better concentration, more positive mood, and so on. Exercising also improves memory by releasing cathepsin B. It’s a protein that triggers the growth of neuron...
with treatment or through gel loading errors, researchers often utilize an antibody that detects the total level of the cognate protein (regardless of phosphorylation state) to determine the phosphorylated fraction relative to the total fraction and to serve as an internal loading control (Figure 1)...