Mucin protein concentration and flow were analyzed by fractionation of the digesta and ethanol precipitation; this process served as the reference method. Alternative methods to estimate MUP consisted of using commercial enzymatic kits to analyze glucosamine (N-acetylglucosamine, GlcNAc) and galactosamine ...
It widely Applied to the separation, purification, concentration of biological macromolecules and desalination, heat removal, etc., and determine the molecular weight of protein Is one of its important applications. Gel is a kind of insoluble bead particles with solid reticular structure and porous ...
ormolecularsievechromatography.ItwidelyAppliedtotheseparation,purification,concentrationofbiologicalmacromoleculesanddesalination,heatremoval,etc.,anddeterminethemolecularweightofproteinIsoneofitsimportantapplications.GelisakindofinsolublebeadparticleswithsolidreticularstructureandporousstructureQuality.Itisusedtoseparatesubstances...
The goal is to reduce the concentration of low molecular weight compounds to a level that will not interfere with subsequent steps in the experiment. A typical dialysis procedure for protein samples is as follows: Pre-wet or prepar...
concentration range of your samples. When large variations in protein concentration need to be analyzed, thePierce Dilution-Free Rapid Gold BCA Assayand theQubit Protein BR Assayoffer the largest dynamic range and are well-suited for s...
One notable advantage of cryo-EM is its ability to determine macromolecular structures in their more close-to-native states and requires a smaller number of samples compared to other structural biology methods5. This is primarily attributed to the plunge freezing method6,7,8, developed four ...
with treatment or through gel loading errors, researchers often utilize an antibody that detects the total level of the cognate protein (regardless of phosphorylation state) to determine the phosphorylated fraction relative to the total fraction and to serve as an internal loading control (Figure 1)...
The dyes used by the QuantiFluor™ RNA System and Quant-iT™ RiboGreen® RNA Reagent are not specific for RNA and will bind to DNA as well. The presence of DNA in an RNA sample may result in an overestimation of nucleic acid concentration. To determine the actual RNA concentration, ...
Cultured meat technology promises to alleviate protein shortages, but still faces many challenges. Here, the authors achieve serum-free myogenic differentiation of porcine pre-gastrulation epiblast stem cells and generate meat-like tissue via edible plant-based scaffolds without any animal compounds. ...
To determine the number of target cells for infection and the amount of virus to use for infection, preliminary dilution experiments were carried out to mimic a library screening method. Considering the total number of kinase cDNAs in the library, dilution experiments were carried out by diluting ...