Methods and apparatus for preparing and assaying biological samples to determine protein concentrationThe present invention discloses methods and apparatus for the detection of biological molecules in various s
It is important to select a kit that covers the expected concentration range of your samples. When large variations in protein concentration need to be analyzed, the Pierce Dilution-Free Rapid Gold BCA Assay and the Qubit Protein BR...
The goal is to reduce the concentration of low molecular weight compounds to a level that will not interfere with subsequent steps in the experiment. A typical dialysis procedure for protein samples is as follows: Pre-wet or prepare...
The ability to determine RNA and proteins subcellular localization, and how they relocalize upon perturbation, is thus key to understanding cellular homeostasis3,4. While cell-wide methods to study protein localization are well established5,6,7, equivalent methods to study RNA subcellular localization...
The aim of broth and agar dilution methods is to determine the lowest concentration of the assayed antimicrobial agent (minimal inhibitory concentration, MIC) that, under defined test conditions, inhibits the visible growth of the bacterium being investi
with treatment or through gel loading errors, researchers often utilize an antibody that detects the total level of the cognate protein (regardless of phosphorylation state) to determine the phosphorylated fraction relative to the total fraction and to serve as an internal loading control (Figure 1)...
It widely Applied to the separation, purification, concentration of biological macromolecules and desalination, heat removal, etc., and determine the molecular weight of protein Is one of its important applications. Gel is a kind of insoluble bead particles with solid reticular structure and porous ...
(1), (2), (3)) were made to determine the protein yield, [protein] being the protein concentration of the given matrix.(1)Protein solubility yield%=100×sup1protein×masssup1biomassprotein×massbiomass(2)Protein precipitation yield%=100×1−sup2protein×masssup2sup1protein×masssup1(3)...
In contrast to LF-MS methods that quantify relative changes in patient samples, both PRM and MRM determine protein levels with absolute quantification. For example, an MRM-MS assay was developed to evaluate α-, β-, and γ-synuclein levels in the CSF of patients with different synucleinopathie...
The high concentration of salt causes the proteins to fall out of solution, and then centrifugation separates the soluble nucleic acid from the cell debris and precipitated protein (1). The DNA is then precipitated by adding isopropanol to the high-concentration salt solution. This forces the ...