METHODS OF DETERMINING PROTEIN OR PEPTIDE CONCENTRATION AND USES THEREOFMethods for determining protein and/or peptide concentration or molecular parameter, such as the extinction coefficient, and uses thereof.Scott Allan BradleyWilliam F. Weiss IV...
If a further decrease in concentration is desired, the dialysis process can be continued with additional volumes of dialysate. Use of dialysis cassette for protein cleanup. 3 mL of 1 mg/mL IgG in 0.1 M Tris buffer, pH 7 inside...
In contrast to other formats for monitoring phosphorylation, ICC is usually the method of choice for determining intracellular localization (View phospho-specific antibodies for ICC/IHC). Both flow cytometry and ICC/IHC require high-affinity and high-specificity antibodies, blocking steps, controls, ...
There are several methods for determining the concentration of protein in samples and reagents. Among them, the BCA, Bradford, and ultraviolet (UV) methods are the most widely used in scientific research. Each method relies on different scientific principles and has its own advantages and di...
Methods for Determining DNA Yield & Purity Determining the yield and purity of isolated DNA is critical to ensure the DNA is usable in downstream applications. DNA yield can be assessed using methods such as absorbance (optical density), agarose gel electrophoresis and fluorescent DNA-binding dyes....
If a further decrease in concentration is desired, the dialysis process can be continued with additional volumes of dialysate. Use of dialysis cassette for protein cleanup. 3 mL of 1 mg/mL IgG in 0.1 M Tris buffer, pH 7 inside...
For more PCR tips, read this blog on the "Top Ten Tips for Successful PCR". Magnesium Concentration Magnesium is a required cofactor for thermostable DNA polymerases, and magnesium concentration is a crucial factor that can affect amplification success. Template DNA concentration, chelating agents ...
Importantly, while messenger RNAs undergo a profound subcellular reorganization upon UPR activation, noncoding RNAs sharing the same properties (for example, size and nucleotide composition) do not, indicating the importance of trans factors in determining RNA localization. Altogether, this uniquely ...
Cryo-electron microscopy (cryo-EM) captures snapshots of dynamic macromolecules, collectively illustrating the involved structural landscapes. This provides an exciting opportunity to explore the structural variations of macromolecules under study. Howev
Determining the class and subclass identity of an antibody is especially important for choosing the method by which it should be purified and used in immunoassays. For example, if an antibody is determined to be IgM, it cannot be purified eff...