In many cases, strongly protected backbone NH sites are neither H-bonded nor buried from solvent, contrary to conventional understanding of HDX. Future studies including OD-and real NH→ND exchange would be informative, but the ab initio simulations required are difficult for large systems.Robert ...
"We really need to understand how these proteins work at a mechanistic level if we're ever going to successfully target them with drugs," said Seth Rubin, assistant professor of chemistry and biochemistry at UC Santa Cruz. "The easiest type of drug to make is one that knocks out a protein...
Polypeptide chain binding proteins: catalysts of protein folding and related processes in cells. How do proteins fold in cells? What are the sequences or structural signals that dictate folding pathways? The new challenge will be to understand ... Rothman,E James - 《Cell》 被引量: 1871发表:...
Explain how enzymes help to control metabolism in living organisms. Be sure to discuss what enzymes are composed of and how their structure affects their function. What is the process of digestion, absorption, and metabolism? Why is it so important to understand the processes of each?
Plant-derived proteins fall into a wide range of scores, from high (like soy), to very low (like sunflower protein or wheat gluten). The FDA has regulations that limit the use of terms like “high,”“rich in,” and “excellent source of,” that are also helpful to understand. In ...
In addition to where a protein is expressed, it's also important to understand how that expression can be altered as a result of your experimental conditions. Let’s say you have quantified the total protein in your samples and are confident you have loaded the same amount across your gel....
To understand how this perturbed chromatin structure upon RSC depletion affects GAL10 transcription dynamics, we used the PP7 technology to directly monitor the transcription in individual cells in real-time (Fig. 1c and Supplementary Video 1). In short, 14 PP7 repeats were introduced endogenously...
These are biologically important questions because the answer to the first two questions could impact our understanding of how protein homeostasis is maintained in cells. The answer to the final question would help to explain how synonymous mutations can have long term impacts on protein structure and...
How to reduce autofluorescenceUnderstand the causes behind autofluorescence and how to deal with this problem.Immunofluorescence (IF) is a popular antibody-based technique, used to analyse the expression and distribution of proteins in tissues and cells. One of the advantages of using IF over ...
Listing a protein’s amino acids is easy. Machines to do so have existed for decades.But this is only half the battle in the quest tounderstand how proteins work. What a protein does, and how it does it, depends also on how itfolds upafter its creation, into its final, intricate shap...