In many cases, strongly protected backbone NH sites are neither H-bonded nor buried from solvent, contrary to conventional understanding of HDX. Future studies including OD-and real NH→ND exchange would be informative, but the ab initio simulations required are difficult for large systems.Robert ...
"We really need to understand how these proteins work at a mechanistic level if we're ever going to successfully target them with drugs," said Seth Rubin, assistant professor of chemistry and biochemistry at UC Santa Cruz. "The easiest type of drug to make is one that knocks out a protein...
Understand the importance of short radius turns, emphasizing their tactical benefits and skills needed to execute them effectively in steeps and moguls. Michael Rogan UpdatedFeb 21, 2024 Instruction Are You Carving or Skidding Down the Hill? Here’s How to Tell ...
In addition to where a protein is expressed, it's also important to understand how that expression can be altered as a result of your experimental conditions. Let’s say you have quantified the total protein in your samples and are confident you have loaded the same amount across your gel. ...
Plant-derived proteins fall into a wide range of scores, from high (like soy), to very low (like sunflower protein or wheat gluten). The FDA has regulations that limit the use of terms like “high,”“rich in,” and “excellent source of,” that are also helpful to understand. In ...
Explain how enzymes help to control metabolism in living organisms. Be sure to discuss what enzymes are composed of and how their structure affects their function. What is the process of digestion, absorption, and metabolism? Why is it so important to understand the processes of each?
These are biologically important questions because the answer to the first two questions could impact our understanding of how protein homeostasis is maintained in cells. The answer to the final question would help to explain how synonymous mutations can have long term impacts on protein structure and...
To understand how this perturbed chromatin structure upon RSC depletion affects GAL10 transcription dynamics, we used the PP7 technology to directly monitor the transcription in individual cells in real-time (Fig. 1c and Supplementary Video 1). In short, 14 PP7 repeats were introduced endogenously...
Listing a protein’s amino acids is easy. Machines to do so have existed for decades.But this is only half the battle in the quest tounderstand how proteins work. What a protein does, and how it does it, depends also on how itfolds upafter its creation, into its final, intricate shap...
Protein coding features can emerge de novo in non coding transcripts, resulting in emergence of new protein coding genes. Studies across many species show that a large fraction of evolutionarily novel non-coding RNAs have an antisense overlap with protei