How reliable are protein structures determined by electron microscopy?Lyman MonroeGenki TerashiDaisuke Kihara
There are 210 thousand experimentally determined protein structures but 250 million known protein sequences. Initiatives like the EarthBioGenome project could generate billions more protein sequences in the next few years. The abundance of data opens the door to applying the approach on an unprecedented...
Key unknowns in chemotaxis are how the motor drives both CCW and CW rotation, how response regulators affect rotation and how torque transfers from the stator to the flagellum. To help inform on these controversies, we determined the structures of the S. enterica serovar typhimurium combined MS-...
How is it determined which mechanism is used? Describe the co-translational sorting of proteins. What organelles are involved? How does the Golgi apparatus deliver proteins to the appropriate location? How are proteins targeted to the correct compartment in a eukaryotic cell? How do the nucleus, ...
In his current paper, Nagano repo.rts on the predictability of a helix, extended chain regions which are potential candi- dates for /3-pleated sheet structures, and looped conforma- tions of the backbone, in proteins of recently determined conformation which do not appear in the data of the ...
The protein concentration of the five plant-based and dairy proteins was determined using the BCA Protein Assay Kit (Thermo Fisher Scientific, IL, USA). Pea P870 (3.39 μg/μl) and pea 1803 (4.18 μg/μl) showed similar values, however casein (8.1 μg/μl), whey 9000 (9.75 μg/μ...
DNA carries all the information for your physical characteristics, which are essentially determined by proteins. DNA contains the instructions for making a protein. In DNA, each protein is encoded by a gene (a specific sequence of DNA nucleotides that specify how a single protein is to be made...
But these microseconds or even hours are negligible compared to the ``Levinthal's time'' of many billions of years required to iterate over all possible structures of the protein chain in attempt to occasionally find the most stable of them. This issue is discussed under 2.4 Levinthal's ...
But only some 170,000 of them have had their structures determined. Dr Moult thinks that boosting this number could help screen drug candidates to see which are likely to bind well to a particular protein. It could be used to reanalyse existing drugs to see what else they might do. And ...
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