crystal structureextracellular matrix proteinsprotein–protein interactionstoichiometryCollagens play important roles in development and homeostasis in most higher organisms. In order to function, collagens require the specific chaperone HSP47 for proper folding and secretion. HSP47 is known to bind to the...
One critical amino acid that determined a conformational neutralizing epitope was identified using mAb 8E4 and PCV2 infectious clone technique. Amino acid residues 47-72 in the capsid protein of PCV2a/CL were replaced with the corresponding region of PCV2b/YJ, and the reactivity of mAb 8E4 ...
The YTHDF proteins are the primary readers of m6A modifications and exert physiological functions of m6A in the cytosol. Elucidating the regulatory mechanisms of YTHDF proteins is critical to understanding m6A biology. Here we report a mechanism that protein post-translational modifications control the ...
Here, we analyzed sequence-dependent effects of sybII and of mutants of sybII on both structure and flexibility of the protein and the interactions with a phospholipid bilayer by means of microsecond atomistic simulations. The sybII TMD was found to direct the folding of both the juxtamembrane ...
inhibition of TGFβ activity abolishes the anabolic activity of mechanical loading16,17, suggesting that TGFβ may also play an essential role in propagating the mechanical signals in AC. TGFβ has been shown to promote chondrocyte proliferation and extracellular matrix (ECM) protein synthesis and re...
The Structure of Eukaryotic Translation Initiation Factor-4E from Wheat Reveals a Novel Disulfide Bond1[OA] The eIF4E crystallized in a dimeric form with one of the cap-binding loops of one monomer inserted into the cavity of the other. The protein also contained an intramolecular disulfide bridge...
A minimal code within Gαo N-terminus governs its compartmentalization and re-coding produces G protein versions with shifted localization. We establish the S-palmitoylation at the outer nuclear membrane assay (“SwissKASH”) to probe substrate specificity of PATs in intact cells. With this assay,...
Cellular sensitivity to ferroptosis is primarily regulated by mechanisms mediating lipid hydroperoxide detoxification. We show that inositol-requiring enzyme 1 (IRE1α), an endoplasmic reticulum (ER) resident protein critical for the unfolded protein res
On the other hand, microglia can also adopt anti-inflammatory functions, and can result in resolution of inflammation, clearance of debris and neural repair. The pro- or anti-inflammatory phenotype is dependent upon the local environment and timing after injury17. Another protein that increases in...
(a) Primary structure of rDc1a. The non-native N-terminal Ser residue that is a vestige of the TEV protease cleavage site used for recombinant toxin production is highlighted in grey. Disulphide bridge connectivity is shown above the sequence. (b) Schematic representation of the pLIC-NSB3 ...