2.5 After finish these steps, then you will get a BioProject numberstart with PRJNAxxx, example number: PRJNA123456 Ⅲ. Uploading the RNAseq rawdata 3.1 Get back to the Submission Portal again, this time click “Sequence Read Archive” 3.2 Click “New submission” 1 SUBMMITER 2 GENERAL INFO...
用RNA-SEQ测一个新的物种的深度测序，然后分析，可以发什么样的文章？？？现在缺5左右的文章 好多SCI...
The authors used mRIN to quantify degradation in large-scale RNA-seq data from postmortem brain tissue in the BrainSpan and Genotype-Tissue Expression (GTEx) projects and found that degradation has a reproducible gene-specific component. This is a preview of subscription content, access via your ...
RNA-seq helps determinegene expressionpatterns based on the number of reads that map to each transcript sequence. It provides comprehensive transcriptome profiling, allowing researchers to detect and quantify the expression of both protein-coding and non-coding RNA molecules. ...
With RNA-seq, the importance of removing unwanted, abundant ribosomal RNA prior to sequencing is often underestimated. Methods that allow researchers to focus on RNA species of interest help them get more useful data at lower cost.
coli K-12 genome by ultra-deep RNA-Seq data. Annotation of genomic positions as antisense ORF, non-coding RNA (ncRNAs) intergenic (IGR), and antisense to ORFs or ncRNAs (AS) was based on gene annotations in the RefSeq and Rfam databases. Positions on the opposite strand of genes were ...
How to Design a good RNA-Seq experiment in an interdisciplinary context ?doi:10.7490/F1000RESEARCH.1096840.1Julie AubertAnne DelafoyeCyprien GuérinChristelle Hennequet-AntierFrédérique HilliouFabrice LegeaiDelphine LabourdetteNathalie MarsaudBrigitte Schaeffer...
Choose 'RNA-Seq' under experimental strategy section Go to 'Files' section on the left-hand side of the page and select 'bam' file under 'Data Format' section Select 'Files' in the main page and add everything (or part of data) into the Cart Click on the Cart (right-corner of the...
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Normally, the sequence data from Whole Transcriptome Sequencing comprises the data for expression profiling as well, and that is why it is the most common form of RNA-Seq projects at the moment. If you simply order an RNA-Seq library prep kit without specifying your objective, you will most...